Phylogenetic relationship of the populations within and around Japan using 105 short tandem repeat polymorphic loci
Phylogenetic relationship of the populations within and around Japan using 105 short tandem repeat polymorphic loci
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DOI：
10.1007/s00439-005-0106-9
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发表时间：
2006-02-01
期刊：
影响因子：
5.3
通讯作者：
Saitou, N
中科院分区：
文献类型：
--
作者：
Li, SL;Yamamoto, T;Saitou, N
我们使用多重PCR分型系统分析了9个东亚和东南亚人群（2个日本人，5个汉人，泰国人和缅甸人）和一个高加索人群的105个常染色体多态性短串联重复（STR）位点。所有STR位点均为全基因组四核苷酸重复标记，在日本人群中观察到的等位基因总数和杂合度分别为756和0.743。对这些等位基因频率数据的系统发育分析表明，日本人群与中国南方人群的亲缘关系比中部和/或北部人群的亲缘关系更密切。结构程序分析表明，在K=2 ~ 6时，两个日本种群的种群结构划分基本清晰，且基本可靠，在K= >= 3时，两个日本种群始终形成一个与其他种群分离的群体，从不属于不同的群体。此外，我们将91个基因座的新等位基因频率数据与先前发表的52个世界人群的等位基因频率数据进行了分析。系统发育和多维尺度（MDS）分析表明，人口规模较大的亚洲人群（6个汉族、3个日本人、2个东南亚人）形成了一个明显的集群，彼此之间的距离比东亚和东南亚其他少数民族更近。在分析STR位点时，这种模式可能是比较种群大小差异很大的种群的鱼子酱。
We have analyzed 105 autosomal polymorphic short tandem repeat (STR) loci for nine East and South-eastern Asian populations (two Japanese, five Han Chinese, Thai, and Burmese populations) and a Caucasian population using a multiplex PCR typing system. All the STR loci are genomewide tetranucleotide repeat markers of which the total number of observed alleles and the observed heterozygosity were 756 and 0.743, respectively, for Japanese populations. Phylogenetic analysis for these allele frequency data suggested that the Japanese populations are more closely related with southern Chinese populations than central and/or northern ones. STRUCTURE program analysis revealed the almost clearly divided and accountable population structure at K=2-6, that the two Japanese populations always formed one group separated from the other populations and never belong to different groups at K >= 3. Furthermore, our new allele frequency data for 91 loci were analyzed with those for 52 worldwide populations published by previous studies. Phylogenetic and multidimensional scaling (MDS) analyses indicated that Asian populations with large population size (six Han Chinese, three Japanese, two Southeast Asia) formed one distinct cluster and are closer to each other than other ethnic minorities in east and Southeast Asia. This pattern may be the caviar of comparing populations with greatly differing population sizes when STR loci were analyzed.