In vivo laminar electrophysiology co-registered with histology in the hippocampus of patients with temporal lobe epilepsy.
In vivo laminar electrophysiology co-registered with histology in the hippocampus of patients with temporal lobe epilepsy.
复制标题
颞叶癫痫患者海马体内层流电生理学与组织学共同记录。
DOI：
10.1016/j.expneurol.2003.12.003
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发表时间：
2004
影响因子：
5.3
通讯作者：
Karmos,George
中科院分区：
文献类型：
--
作者：
Ulbert,István;Maglóczky,Zsófia;Eross,Loránd;Czirják,Sándor;Vajda,János;Bognár,László;Tóth,Szabolcs;Szabó,Zerind;Halász,Péter;Fabó,Dániel;Halgren,Eric;Freund,TamásF;Karmos,George
层状多微电极已经被开发用于对动物的皮层和海马活动进行采样。如果这些测量结果与该区域的解剖结构充分配准，它们可以产生关于其功能和结构的重要信息。在体内层状电生理记录从人类癫痫海马是罕见的。然而，组织学和免疫组织化学分析被广泛用于确定与颞叶癫痫（TLE）相关的结构变化。在这里，我们提出了一个综合的方法获得的数据：术中记录层流场电位，单和多个单位的活动麻醉下，伴随着组织学和免疫组织化学从同一海马区的癫痫患者进行颞叶切除术耐药TLE。成功地测试了电生理的稳定性及其与组织学配准的准确性。我们已经发现了大的场电位尖峰与CA1中的单个单元的爆发。皮质内和硬膜下带状录音从外侧颞叶皮层表现出类似的场电位激活模式。一个突出的振荡活动是目前在齿状回具有高度本地化的场电位梯度和多个单位的活动。该图案可以用作限定电极在海马体中的位置的标志。我们的研究结果表明，海马结构的局部和网络癫痫样活动的某些方面可能在麻醉下保留。对海马功能状态及其局部结构相关性的电生理学鉴定可以进一步增强我们对这种疾病的理解。
Laminar multiple microelectrodes have been developed to sample cortical and hippocampal activity in animals. If these measurements are adequately co-registered with the anatomy of the region, they can yield important information about its function and structure. In vivo laminar electrophysiological recordings from the human epileptic hippocampus are rare. However, histological and immunohistochemical analyses are widely used to determine the structural changes associated with temporal lobe epilepsy (TLE). Here we present data obtained by a combined approach: intraoperative recording of laminar field potentials, single and multiple unit activity under anesthesia, accompanied by histology and immunohistochemistry from the same hippocampal region of epileptic patients undergoing temporal lobectomy for drug-resistant TLE. The stability of the electrophysiology and the accuracy of its co-registration with histology were tested successfully. We have found large field potential spikes associated with bursting single units in CA1. Intracortical and subdural strip recordings from the lateral temporal cortex showed similar field potential activation patterns. A prominent oscillatory activity was present in the dentate gyrus with highly localized field potential gradient and multiple unit activity. This pattern could be used as a landmark defining the position of the electrode in the hippocampus. Our findings indicate that some aspects of the local and network epileptiform activity in the hippocampal formation are likely preserved under anesthesia. Electrophysiological identification of the functional state of the hippocampus together with its local structural correlates could further enhance our understanding of this disease.