A new radioimmunoassay for plasma L-triiodothyronine: measurements in thyroid disease and in patients maintained on hormonal replacement.
A new radioimmunoassay for plasma L-triiodothyronine: measurements in thyroid disease and in patients maintained on hormonal replacement.
复制标题
血浆 L-三碘甲状腺原氨酸的新放射免疫测定法：甲状腺疾病和维持激素替代的患者的测量。
DOI：
--
复制
发表时间：
1972
影响因子：
15.9
通讯作者：
J. Oppenheimer
中科院分区：
文献类型：
--
作者：
M. Surks;A. R. Schadlow;J. Oppenheimer
本文描述了一种新的放射免疫测定人血浆中l-三碘甲状腺原氨酸（T(3)）的方法，在与T(3)特异性抗血清孵育之前，将碘甲状腺原氨酸从血浆蛋白中分离出来。结合抗体和游离T(3)用葡聚糖包被炭分离。在该系统中，添加到血浆中的T(3)的平均回收率为97.9%，l-甲状腺素（T(4)）向T(3)的体外转化以及T(4)与抗T(3)抗体的交叉反应均未检测到（小于0.1%）。该检测程序允许在0.5 ml血浆中测量T(3)，从而提高检测灵敏度（6 ng/100 ml）。正常人的平均血浆T(3)为146+/-24 ng/100 ml （sd）。甲状腺机能亢进组平均T(3)浓度升高（665+/-289 ng/100 ml），甲状腺机能减退组平均T(3)浓度降低（44+/-26 ng/100 ml）。在严重甲状腺功能减退患者中，血浆T(3)在7 - 30 ng/100 ml之间。在三名甲状腺功能正常的受试者中，血浆T(3)浓度全天相对稳定。相反，在甲状腺功能减退的患者中，在服用T(3)替代疗法后，T(4): T(3)组合或干燥的甲状腺血浆T(3)在摄入药物后数小时内显著升高。血浆T(3)在接受T(4)治疗的患者中全天保持不变。因此，就血浆T(3)水平而言，T(4)替代疗法似乎比其他制剂更接近于模拟甲状腺功能正常状态。
A new procedure for the radioimmunoassay of l-triiodothyronine (T(3)) in human plasma is described in which the iodothyronines are separated from the plasma proteins before incubation with a specific antiserum to T(3). The antibody bound and free T(3) are separated with dextran-coated charcoal. In this system, the mean recovery of T(3) added to plasma was 97.9% and both in vitro conversion of l-thyroxine (T(4)) to T(3) and cross-reaction between T(4) and the anti-T(3) antibody were undetectable (less than 0.1%). The assay procedure allowed the measurement of T(3) in up to 0.5 ml of plasma resulting in improved assay sensitivity (6 ng/100 ml). The mean plasma T(3) in normal subjects was 146+/-24 ng/100 ml (sd). Mean T(3) concentration was increased in hyperthyroidism (665+/-289 ng/100 ml) and decreased in hypothyroidism (44+/-26 ng/100 ml). In patients with severe hypothyroidism, plasma T(3) was between 7 and 30 ng/100 ml. Plasma T(3) concentration was relatively constant throughout the day in three euthyroid subjects. In contrast, in hypothyroid subjects on replacement therapy with T(3), a T(4): T(3) combination or desiccated thyroid plasma T(3) was markedly elevated for several hours after ingestion of the medication. Plasma T(3) was unchanged throughout the day in patients treated with T(4). Thus, insofar as plasma T(3) levels are concerned, replacement therapy with T(4) appears to mimic the euthyroid state more closely than other preparations.