tRNAscan-SE On-line: integrating search and context for analysis of transfer RNA genes.
tRNAscan-SE On-line: integrating search and context for analysis of transfer RNA genes.
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DOI：
10.1093/nar/gkw413
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发表时间：
2016-07-08
影响因子：
14.9
通讯作者：
Chan PP
中科院分区：
文献类型：
--
作者：
Lowe TM;Chan PP
高通量基因组测序使得对快速、准确的基因组注释的需求不断增加，而tRNA基因构成了必需的、始终存在的非编码RNA基因中最大的家族。新开发的tRNAscan - SE 2.0提升了tRNA基因检测和功能预测方面的最先进方法，其相关的基因组tRNA数据库有丰富的新内容。以前，网络服务器对tRNA的检测与对现有tRNA及其注释的了解是脱节的。在这次tRNAscan - SE在线资源的更新中，我们通过网络服务器搜索结果、GtRNAdb中最相关的基因以及UCSC基因组浏览器提供的交互式丰富基因组环境之间动态生成的链接，将tRNA分类的改进与极大增强的生物学背景联系起来。tRNAscan - SE在线网络服务器可通过http://trna.ucsc.edu/tRNAscan - SE/访问。
High-throughput genome sequencing continues to grow the need for rapid, accurate genome annotation and tRNA genes constitute the largest family of essential, ever-present non-coding RNA genes. Newly developed tRNAscan-SE 2.0 has advanced the state-of-the-art methodology in tRNA gene detection and functional prediction, captured by rich new content of the companion Genomic tRNA Database. Previously, web-server tRNA detection was isolated from knowledge of existing tRNAs and their annotation. In this update of the tRNAscan-SE On-line resource, we tie together improvements in tRNA classification with greatly enhanced biological context via dynamically generated links between web server search results, the most relevant genes in the GtRNAdb and interactive, rich genome context provided by UCSC genome browsers. The tRNAscan-SE On-line web server can be accessed at http://trna.ucsc.edu/tRNAscan-SE/.