Monitoring of intercellular messengers released from neuron networks cultured in a microchip.
Monitoring of intercellular messengers released from neuron networks cultured in a microchip.
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DOI：
10.1016/j.chroma.2005.06.099
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发表时间：
2006-04
期刊：
影响因子：
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通讯作者：
Kiichi Sato;Akiko Egami;T. Odake;M. Tokeshi;M. Aihara;T. Kitamori
中科院分区：
文献类型：
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作者：
Kiichi Sato;Akiko Egami;T. Odake;M. Tokeshi;M. Aihara;T. Kitamori
细胞生化分析系统集成在石英玻璃微芯片上，微室用于细胞培养，微通道用于热透镜显微镜(TLM)检测。在该芯片中成功地培养了大鼠海马神经细胞形成神经网络。谷氨酸的水溶液被认为是一种神经递质，用来刺激培养的神经元释放逆行信使花生四烯酸，花生四烯酸被认为是神经元可塑性的关键，特别是对长时程增强(LTP)。介绍完后，用UV-TLM(激发波长，244 nm)对流经培养箱的溶液进行分析。测量的信号强度依赖于谷氨酸溶液的浓度，并认为神经元根据谷氨酸浓度释放逆行信使。该系统适用于从极少量的培养细胞中释放超微量化学物质的时间过程监测。
A cellular biochemistry analysis system was integrated on a quartz glass microchip with a microchamber for cell culture followed by a microchannel for detecting with a thermal lens microscope (TLM). Nerve cells from rat hippocampus were successfully cultured to form neural networks in the microchip. An aqueous solution of glutamate, which is known as a neurotransmitter, was introduced to stimulate the cultured neuron to release a retrograde messenger, arachidonate which is considered to be critical for neuronal plasticity, especially for long-term potentiation (LTP). After the introduction, the solution that flowed through the culture chamber was analyzed using the UV-TLM (excitation wavelength, 244nm). The measured signal intensity was dependent on glutamate solution concentration, and the neurons were considered to release the retrograde messenger according to the glutamate concentration. This system is suitable for time-course monitoring of ultra trace amounts of chemicals released from very small amount of cultured cells.