Surface modification chemistry based on the electrostatic adsorption of poly-L-arginine onto alkanethiol modified gold surfaces
Surface modification chemistry based on the electrostatic adsorption of poly-L-arginine onto alkanethiol modified gold surfaces
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DOI：
10.1021/la035158m
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发表时间：
2003-11-25
期刊：
影响因子：
3.9
通讯作者：
Silva, F
中科院分区：
文献类型：
--
作者：
Barreira, SVP;Silva, F
精氨酸的胍基与α -二羰基官能团之间的反应被用来开发一种新的表面修饰化学。第一步是将聚l -精氨酸层静电吸附在可电离的烷硫醇修饰的金表面上。精氨酸残基（pK(a) > 12）的胍基的强碱性保证了多肽在带负电荷的金表面上的牢固附着，直到非常高的pH值。通过改变聚l -精氨酸静电吸附溶液的pH值，可以控制沉积量。证明了聚l -精氨酸层的表面胍基可以用α -二羰基反应探针进一步衍生，产生稳定的杂环缩合物。此外，与杂双功能试剂对叠氮苯基乙二醛（APG）的反应提供了一个以光敏芳基叠氮基团末端的表面，用于光化学固定蛋白质到表面。证明了这种表面修饰化学在固定抗体上的应用。
The reaction between the arginine's guanidino group and alpha-dicarbonyl functionalities was used to develop a novel surface modification chemistry. The first modification step consists of the electrostatic adsorption of a poly-L-arginine layer onto ionizable alkanethiol modified gold surfaces. The strongly basic character of the guanidino group of the arginine residues (pK(a) > 12) guarantees the robust attachment of the polypeptide to negatively charged gold surfaces until very high pH. By varying the pH of the solution from which poly-L-arginine is electrostatically adsorbed, it is possible to control the amount deposited. The availability of the surface guanidino groups of the poly-L-arginine layer for further derivatization with alpha-dicarbonyl reaction probes, yielding stable heterocyclic condensation adducts, is demonstrated. In addition, the reaction with the heterobifunctional reagent p-azidophenyl glyoxal (APG) provides a surface terminated with a photosensitive aryl azide group which was employed for the photochemical immobilization of proteins to the surface. The application of this surface modification chemistry to immobilize antibodies is demonstrated.