AN EXTRACELLULAR-MATRIX PROTEIN IN PLANTS - CHARACTERIZATION OF A GENOMIC CLONE FOR CARROT EXTENSIN
AN EXTRACELLULAR-MATRIX PROTEIN IN PLANTS - CHARACTERIZATION OF A GENOMIC CLONE FOR CARROT EXTENSIN
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DOI：
10.1002/j.1460-2075.1985.tb03908.x
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发表时间：
1985-01-01
期刊：
影响因子：
11.4
通讯作者：
VARNER, JE
中科院分区：
文献类型：
--
作者：
CHEN, J;VARNER, JE
伸展蛋白是一种富含羟基脯氨酸的糖蛋白，存在于许多植物细胞壁中，是一种主要的蛋白质成分。延伸蛋白中含有丰富的ser - hypp - hypp - hypp - hypp肽。以延伸蛋白cDNA克隆为探针，从胡萝卜基因组文库中分离出6个不同的克隆。其中一个基因组克隆pDC5A1被鉴定并发现包含一个编码扩展蛋白的开放阅读框和一个位于3‘’非编码区的内含子。衍生的氨基酸序列包含1个肽序列和25个ser - pro - pro - pro重复序列。发现两个扩展蛋白转录本对应于pDC5A1，具有不同的5'起始位点。这些转录本在受伤后数量增加。这与报道的损伤后细胞壁的伸展蛋白积累一致。
Extensins are hydroxyproline-rich glycoproteins found in many plant cell walls as a major protein component. The peptide Ser-Hyp-Hyp-Hyp-Hyp is abundant in the extensins. Using extensin cDNA clones as probes, we isolated six different clones from carrot genomic libraries. One of the genomic clones, pDC5A1, was characterized and found to contain an open reading frame encoding extensin and a single intron in the 3''-non-coding region. The derived amino acid sequence contains a single peptide sequence and 25 Ser-Pro-Pro-Pro-Pro repetitive sequences. Two extensin transcripts were found corresponding to pDC5A1 with different 5'' start sites. These transcripts increase in abundance after wounding. This is consistent with the reported extensin accumulation in the cell wall upon wounding.