SOURCES OF AMMONIA FOR MAMMALIAN UREA SYNTHESIS
SOURCES OF AMMONIA FOR MAMMALIAN UREA SYNTHESIS
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DOI：
10.1042/bj1760733
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发表时间：
1978-01-01
影响因子：
4.1
通讯作者：
READ, WWC
中科院分区：
文献类型：
--
作者：
KREBS, HA;HEMS, R;READ, WWC
在大鼠肝细胞中，[15 N]丙氨酸掺入腺嘌呤核苷酸的6-氨基的初始速率约为掺入尿素的速率的1/18。因此，嘌呤核苷酸循环不能提供氨甲酰磷酸合酶反应尿素合成中所需的大部分氨（EC 2.7.2.5）。另一方面，与McGivan和Chappell表达的观点相反，实验支持肝谷氨酸脱氢酶可以提供所需氨的观点。
The initial rate of incorporation of [15N]alanine into the 6-amino group of the adenine nucleotides in rat hepatocytes was about 1/18 of the rate of incorporation into urea. Thus the purine nucleotide cycle cannot provide most of the ammonia needed in urea synthesis for the carbamoyl phosphate synthase reaction (EC 2.7.2.5). On the other hand, contrary to the view expressed by McGivan and Chappell, the experiments support the view that hepatic glutamate dehydrogenase can supply the required ammonia.