Identification of candidate periodontal pathogens and beneficial species by quantitative 16S clonal analysis
Identification of candidate periodontal pathogens and beneficial species by quantitative 16S clonal analysis
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DOI：
10.1128/jcm.43.8.3944-3955.2005
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发表时间：
2005-08-01
影响因子：
9.4
通讯作者：
Leys, EJ
中科院分区：
文献类型：
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作者：
Kumar, PS;Griffen, AL;Leys, EJ
大多数关于牙周炎细菌病因的研究要么使用基于培养的方法，要么使用靶向DNA方法，因此很可能病原体仍未被发现。本研究的目的是利用与慢性牙周炎和牙周健康相关的生物膜的培养独立的定量分析来鉴定病原体和有益物种。采用核糖体16S克隆和测序技术对牙周炎患者和对照组的样本进行分析。有几个属与牙周炎有关，其中许多属未经培养，其中革兰氏阳性最多，包括胃链球菌和丝状杆菌。在牙周炎中，Megasphaera属和Desulfobulbus属升高，弯曲杆菌、硒单胞菌、脱铁杆菌、Dialister、Catonella、Tannerella、链球菌、Atopobium、真杆菌和密螺旋体等几种种或种型的水平升高。在所有样本中都发现了大量的链球菌和细络菌，并且在健康受试者的微生物群落中所占的比例明显高于牙周炎患者。牙周健康的微生物谱还包括较少的弯曲杆菌属，无营养菌属，Gemella，嗜碳细胞菌属和奈瑟菌属。这些新发现的候选菌种数量超过了牙龈卟啉单胞菌和其他先前被认为是牙周病致病菌的菌种，目前尚不清楚新发现的数量更多的菌种是否在发病机制中发挥更重要的作用。最后，在牙周炎受试者和健康受试者之间发现的细菌谱差异大于同一受试者体内深层和浅层部位之间的差异。这表明慢性牙周炎是口腔细菌生态全局扰动的结果，而不是疾病部位特异性微生物转移的结果。
Most studies of the bacterial etiology of periodontitis have used either culture-based or targeted DNA approaches, and so it is likely that pathogens remain undiscovered. The purpose of this study was to use culture-independent, quantitative analysis of biofilms associated with chronic periodontitis and periodontal health to identify pathogens and beneficial species. Samples from subjects with periodontitis and controls were analyzed using ribosomal 16S cloning and sequencing. Several genera, many of them uncultivated, were associated with periodontitis, the most numerous of which were gram positive, including Peptostreptococcus and Filifactor. The genera Megasphaera and Desulfobulbus were elevated in periodontitis, and the levels of several species or phylotypes of Campylobacter, Selenomonas, Deferribacteres, Dialister, Catonella, Tannerella, Streptococcus, Atopobium, Eubacterium, and Treponema were elevated in disease. Streptococcus and Veillonella spp. were found in high numbers in all samples and accounted for a significantly greater fraction of the microbial community in healthy subjects than in those with periodontitis. The microbial profile of periodontal health also included the less-abundant genera Campylobacter, Abiotrophia, Gemella, Capnocytophaga, and Neisseria. These newly identified candidates outnumbered Porphyromonas gingivalis and other species previously implicated as periodontopathogens, and it is not clear if newly identified and more numerous species may play a more important role in pathogenesis. Finally, more differences were found in the bacterial profile between subjects with periodontitis and healthy subjects than between deep and shallow sites within the same subject. This suggests that chronic periodontitis is the result of a global perturbation of the oral bacterial ecology rather than a disease-site specific microbial shift.