An evaluation of white matter injury after spinal cord ischemia in rats: A comparison with gray matter injury
An evaluation of white matter injury after spinal cord ischemia in rats: A comparison with gray matter injury
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DOI：
10.1213/01.ane.0000146523.56647.5e
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发表时间：
2005-03-01
影响因子：
5.7
通讯作者：
Furuya, H
中科院分区：
文献类型：
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作者：
Kurita, N;Kawaguchi, M;Furuya, H
我们定量评估大鼠脊髓缺血后灰质和白色物质损伤，并确定灰质和白色物质损伤程度之间的关系。雄性大鼠25只，异氟醚麻醉，球囊主动脉阻断联合降压诱导脊髓缺血（SCI）。将动物随机分配到以下三组之一：SCI 12 min（SCI-12; n = 8），15 min（SCI-15; n = 9）或假手术组（n = 8）。再灌注后24小时，采用Basso-Beattie-Bresnahan量表评分评估后肢运动功能。根据前角中正常神经元的数量评估灰质损伤。根据腹侧和腹外侧白色物质中空泡化和淀粉样前体蛋白免疫反应性的程度评估白色物质损伤。SCI-15组正常神经元数量明显少于SCI-12组和Sham组（P < 0.05）。Basso-Beattie-Bresnahan评分与正常神经元数量呈显著正相关。SCI-15组的空泡化面积百分比显著大于SCI-12组和假手术组（30% +/- 10%对9% +/-7%，0% +/-0%，P < 0.05）。免疫组化分析显示，淀粉样前体蛋白免疫反应性增加的肿胀轴突，特别是在SCI-15组。正常神经元数目与空泡化面积百分比呈显著负相关。这些结果表明，大鼠脊髓损伤后，灰质和白色物质都受到损伤，并且在相对较短的恢复期后，白色物质损伤的程度与灰质损伤的严重程度相关。
We quantitatively assessed both gray and white matter injury after spinal cord ischemia in rats, and the relationship between the magnitude of gray and white matter injury was determined. Twenty-five male rats were anesthetized with isoflurane, and spinal cord ischemia (SCI) was induced by balloon intraaortic occlusion combined with hypotension. The animals were randomly allocated to one of the following three groups: animals with SCI for 12 min (SCI-12; n = 8), 15 min (SCI-15; n = 9), or those with sham operation (n = 8). Twenty-four hours after reperfusion, hindlimb motor function was assessed using the Basso-Beattie-Bresnahan scale scoring. Gray matter damage was assessed on the basis of the number of normal neurons in the ventral horn. White matter damage was assessed on the basis of the extent of vacuolation and amyloid precursor protein immunoreactivity in the ventral and ventrolateral white matter. There were significantly less normal neurons in the SCI-15 group compared with those in the SCI-12 and sham groups (P < 0.05). There was a significant positive correlation between the Basso-Beattie-Bresnahan scores and the number of normal neurons. The percentages of vacuolation areas in the SCI-15 group were significantly larger compared with those in the SCI-12 and sham groups (30% +/- 10% versus 9% +/- 7%, 0% +/- 0%, P < 0.05). Immunohistochemical analysis revealed increased amyloid precursor protein immunoreactivity in the swollen axons, especially in the SCI-15 group. There was a significant negative correlation between the number of normal neurons and percentages of vacuolation areas. These results indicate that both gray and white matter were injured after SCI in rats and the degree of white mater injury was correlated with the severity of gray matter injury after a relatively short recovery period.