Effects of renin gene transfer on blood pressure and renin gene expression in a congenic strain of Dahl salt-resistant rats.
Effects of renin gene transfer on blood pressure and renin gene expression in a congenic strain of Dahl salt-resistant rats.
复制标题
肾素基因转移对达尔耐盐大鼠同系品系血压和肾素基因表达的影响。
DOI：
10.1172/jci118444
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Kurtz,TW
中科院分区：
文献类型：
--
作者：
StLezin,EM;Pravenec,M;Wong,AL;Liu,W;Wang,N;Lu,S;Jacob,HJ;Roman,RJ;Stec,DE;Wang,JM;Reid,IA;Kurtz,TW
为了研究在大鼠13号染色体肾素基因座附近是否存在BP调节基因，我们将这段来自Dahl盐敏感(S)大鼠的染色体片段转移到Dahl耐盐(R)大鼠的遗传背景上。携带S肾素基因的同源Dahl R大鼠在饲喂8%的食盐后，其收缩压显著低于其祖先Dahl R大鼠：分别为12 7+/-1 mm Hg和138+/-4 mm Hg(P&t；0.0 5)。此外，同源Dahl R品系的血压降低与肾脏肾素mRNA的降低和血浆肾素浓度的降低有关。这些发现表明，与Dahl R品系的相应等位基因相比，Dahl R品系在肾素基因座或其附近携带有较低的血浆肾素浓度和较低的BP，至少在Dahl R大鼠的遗传背景和高盐饮食环境中是这样。在自然发生的肾素基因变异株间转移后，肾脏肾素mRNA、血浆肾素浓度和血压同时降低，强烈表明肾素基因表达的遗传变异可以影响血压。
To investigate whether a BP-regulatory locus exists in the vicinity of the renin locus on rat chromosome 13, we transferred this chromosome segment from the Dahl salt-sensitive (S) rat onto the genetic background of the Dahl salt-resistant (R) rat. In congenic Dahl R rats carrying the S renin gene and fed an 8% salt diet, systolic BP was significantly lower than in progenitor Dahl R rats: 127 +/- 1 mmHg versus 138 +/- 4 mmHg, respectively (P < 0.05). Moreover, the decreased BP in the congenic Dahl R strain was associated with decreased kidney renin mRNA and decreased plasma renin concentration. These findings demonstrate that the Dahl S strain carries alleles in or near the renin locus that confer lower plasma renin concentration and lower BP than the corresponding alleles in the Dahl R strain, at least when studied on the genetic background of the Dahl R rat and in the environment of a high salt diet. The occurrence of coincident reductions in kidney renin mRNA, plasma renin concentration, and BP after interstrain transfer of naturally occurring renin gene variants strongly suggests that genetically determined variation in renin gene expression can affect BP.