Frequencies of paired open-closed durations of ion channels. Method of evaluation from single-channel recordings.
Frequencies of paired open-closed durations of ion channels. Method of evaluation from single-channel recordings.
复制标题
离子通道成对开闭持续时间的频率。
DOI：
10.1016/s0006-3495(87)83187-9
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发表时间：
1987
影响因子：
3.4
通讯作者：
I. Z. Steinberg
中科院分区：
文献类型：
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作者：
I. Z. Steinberg
在单通道记录中，连续的闭-开或开-闭间隔在特定持续时间内出现的概率，对于建立描述通道行为的动力学模型可能有巨大的帮助。相应的概率函数是闭持续期和开持续期的指数函数乘积的线性组合。提出了一种利用一组辅助函数来计算指数函数系数的方法，这些辅助函数除了一个之外都与所有指数函数正交。然后，通过乘以辅助函数和随后的简单积分运算，可以从实验数据中获得概率函数中的系数。此外，由于沟道电导中的转变的随机性质，通过使用上述辅助函数，也容易估计在参数的评估的数值幅度中预期的方差。通过计算机模拟实验得到的合成数据的分析，说明了该过程。
The probability of the occurrence of consecutive closed-open or open-closed intervals of specified durations in single-channel recordings may be of enormous help in the establishment of the kinetic scheme that describes the behavior of the channel. The relevant probability functions are linear combinations of products of exponential functions of the closed durations and the open durations. A method is presented for the evaluation of the coefficients of the exponential functions using a set of auxiliary functions that are each orthogonal to all but one of the exponential functions. The coefficients in the probability functions may then be obtained from the experimental data by multiplication by the auxiliary functions and subsequent simple integration operations. Furthermore, the variance to be expected in the evaluated numerical magnitude of the parameters, due to the stochastic nature of the transitions in the channel conductance, is also readily estimated by use of the above auxiliary functions. The procedure is illustrated by analysis of synthetic data obtained from computer simulated experiments.