Insights from an erroneous kinetochore-microtubule attachment state.
Insights from an erroneous kinetochore-microtubule attachment state.
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DOI：
10.4161/bioa.25734
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发表时间：
2013-05
期刊：
影响因子：
--
通讯作者：
Maresca TJ
中科院分区：
文献类型：
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作者：
Cane S;McGilvray PT;Maresca TJ
基因组的忠实分布需要在细胞分裂期间聚集在每个染色单体上的姐妹动粒与来自相反纺锤体两极的动态微管相互作用，这种配置称为染色体双向定位。双取向产生张力，通过不明确的机制增加微管的动粒的亲和力。非双向激动素-微管（kt-MT）相互作用很普遍，但由于错误纠正途径降低了激动素对微管的亲和力，因此持续时间很短。有趣的是，不正确的kt-MT相互作用可以通过实验性地施加力到错误定向的染色体上来稳定。在这里，利用活细胞力测定来表征常见类型的不当kt-MT附着的分子组成。我们的力相关的研究也讨论了在当前的背景下，张力依赖稳定的kt-MT相互作用的模型。
Faithful distribution of the genome requires that sister kinetochores, which assemble on each chromatid during cell division, interact with dynamic microtubules from opposite spindle poles in a configuration called chromosome biorientation. Biorientation produces tension that increases the affinity of kinetochores for microtubules via ill-defined mechanisms. Non-bioriented kinetochore-microtubule (kt-MT) interactions are prevalent but short-lived due to an error correction pathway that reduces the affinity of kinetochores for microtubules. Interestingly, incorrect kt-MT interactions can be stabilized by experimentally applying force to misoriented chromosomes. Here, a live-cell force assay is utilized to characterize the molecular composition of a common type of improper kt-MT attachment. Our force-related studies are also discussed in the context of current models for tension-dependent stabilization of kt-MT interactions.