Gemin5: A Multitasking RNA-Binding Protein Involved in Translation Control.
Gemin5: A Multitasking RNA-Binding Protein Involved in Translation Control.
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DOI：
10.3390/biom5020528
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发表时间：
2015-04-17
期刊：
影响因子：
5.5
通讯作者：
Martinez-Salas E
中科院分区：
文献类型：
--
作者：
Piñeiro D;Fernandez-Chamorro J;Francisco-Velilla R;Martinez-Salas E
GINE5是一种RNA结合蛋白(RBP)，最初被鉴定为运动神经元存活(SMN)复合体的外周成分。这种以细胞质为主的蛋白质通过其WD重复结构域识别小核RNA(SnRNAs)，允许SMN复合体组装成小核核糖核蛋白(SnRNPs)。此外，据报道，该蛋白的氨基末端具有帽子结合能力，并与真核细胞起始因子4E(EIF4E)相互作用。Gin5还被证明下调翻译，是微小核糖核酸病毒L蛋白酶的底物，并通过位于其羧基末端的两部分非正则结合位点与病毒内部核糖体进入位点元件相互作用。这些功能将Gin5与翻译控制事件联系起来。因此，除了在SNRNPs生物发生中的作用外，Gin5似乎是一个多任务蛋白质，在各种RNA引导的过程中合作。在这篇综述中，我们将总结当前关于双子座5功能的知识。我们将讨论蛋白质在翻译控制中的作用，并提出一个模型来解释微小核糖核酸病毒感染细胞中这种RBP的蛋白分解片段如何调节蛋白质的合成。
Gemin5 is a RNA-binding protein (RBP) that was first identified as a peripheral component of the survival of motor neurons (SMN) complex. This predominantly cytoplasmic protein recognises the small nuclear RNAs (snRNAs) through its WD repeat domains, allowing assembly of the SMN complex into small nuclear ribonucleoproteins (snRNPs). Additionally, the amino-terminal end of the protein has been reported to possess cap-binding capacity and to interact with the eukaryotic initiation factor 4E (eIF4E). Gemin5 was also shown to downregulate translation, to be a substrate of the picornavirus L protease and to interact with viral internal ribosome entry site (IRES) elements via a bipartite non-canonical RNA-binding site located at its carboxy-terminal end. These features link Gemin5 with translation control events. Thus, beyond its role in snRNPs biogenesis, Gemin5 appears to be a multitasking protein cooperating in various RNA-guided processes. In this review, we will summarise current knowledge of Gemin5 functions. We will discuss the involvement of the protein on translation control and propose a model to explain how the proteolysis fragments of this RBP in picornavirus-infected cells could modulate protein synthesis.