Quantitative genetics of vector competence for La Crosse virus and body size in Ochlerotatus hendersoni and Ochlerotatus triseriatus interspecific hybrids
Quantitative genetics of vector competence for La Crosse virus and body size in Ochlerotatus hendersoni and Ochlerotatus triseriatus interspecific hybrids
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DOI：
10.1534/genetics.104.033639
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发表时间：
2005-03-01
期刊：
影响因子：
3.3
通讯作者：
Severson, DW
中科院分区：
文献类型：
--
作者：
Anderson, JR;Schneider, JR;Severson, DW
拉克罗斯病毒是美国儿童脑炎的主要病因。三列Ochterolatus是拉克罗斯病毒的有效载体，而与之密切相关的三列Ochterolatus是拉克罗斯病毒的有效载体。Hendersoni仅以非常低的速率传输。本研究对164个来自稻瘟病菌种间杂交的雌性个体进行了影响口传能力和成虫体型大小的数量性状基因座（QTL）定位。hendersoni雌虫和O.利用复合区间作图法（CIM）、二元性状的区间作图法（IM）和单标记作图法对三列绒螯蟹雄性群体进行了定位。对于口腔传播（OT），没有基因组位置超过95%的实验阈值宣布一个QTL使用IM，但单标记分析确定了四个独立的区域显着相关的OT，我们认为是暂定QTL。对于两个QTL，OT的增加与来自难治性载体O的等位基因相关。hendersoni，可能反映了我们种间杂交发现的基因之间的上位相互作用。对于身体大小，两个QTL被确定使用CIM和第三个暂定QTL被确定使用单标记分析。与身体大小相关的基因组区域也包含三个控制OT的QTL，这表明这些区域包含具有多效性效应的单基因或独立决定每个性状的多个连锁基因。
La Crosse virus is a leading cause of pediatric encephalitis in the United States. The mosquito Ochterolatus triseriatus is an efficient vector for La Crosse virus, whereas the closely related O. hendersoni transmits only at very low rates. Quantitative trait loci (QTL) affecting the ability to orally transmit this virus and adult body size were identified in 164 F, female individuals from interspecific crosses of O. hendersoni females and O. triseriatus males rising a combination of composite interval mapping (CIM), interval mapping (IM) for binary traits, and single-marker mapping. For oral transmission (OT), no genome locations exceeded the 95% experimentwise threshold for declaring a QTL using IM, but single-marker analysis identified four independent regions significantly associated with OT that we considered as tentative QTL. With two QTL, an increase in OT was associated with alleles from the refractory vector, O. hendersoni, and likely reflect epistatic interactions between genes that were uncovered by our interspecific crosses. For body size, two QTL were identified using CIM and a third tentative QTL was identified using single-marker analysis. The genome regions associated with body size also contain three QTL controlling OT, suggesting that these regions contain either single genes with pleiotropic effects or multiple linked genes independently determining each trait.