Cytosolic LOX overexpression in Arabidopsis enhances the attractiveness of parasitic wasps in response to herbivory and incidences of parasitism
Cytosolic LOX overexpression in Arabidopsis enhances the attractiveness of parasitic wasps in response to herbivory and incidences of parasitism
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DOI：
10.1080/17429145.2013.772663
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发表时间：
2013-09-01
影响因子：
3.2
通讯作者：
Takabayashi, Junji
中科院分区：
文献类型：
--
作者：
Ozawa, Rika;Shiojiri, Kaori;Takabayashi, Junji
为了应对菜粉蝶 (Pieris rapae) 幼虫的食草行为，十字花科植物会释放出绿叶挥发物 (GLV)，吸引幼虫的肉食性天敌 Cotesia glomerata。为了人为地增加十字花科植物的 GLV 产量，我们创建了组成型表达黄瓜胞质脂氧合酶 (CsLOX2) 的转基因拟南芥。感染了菜籽油菜幼虫的转基因拟南芥（p35S::CsLOX2.6 和 p35S::CsLOX2.14）比野生型（wt）对球花菜更具吸引力，并产生更多的茉莉酸。 p35S::CsLOX2.14 的幼虫寄生发生率高于 wt。受感染的转基因植物比受感染的野生植物释放更多的(Z)-3-己烯基乙酸酯、(E)-罗勒烯、(Z)-罗勒烯和DMNT；众所周知，(Z)-3-己烯基乙酸酯可以吸引 Cotesia 黄蜂。用胞质 CsLOX2 转化十字花科作物可以通过三营养相互作用帮助综合害虫治理。
In response to herbivory by cabbage white butterfly (Pieris rapae) larvae, crucifer plants emit green leaf volatiles (GLVs) that attract Cotesia glomerata, a carnivorous natural enemy of the larvae. To artificially increase GLV production by crucifers, we created transgenic Arabidopsis that constitutively expressed cucumber cytosolic lipoxygenase (CsLOX2). Transgenic Arabidopsis (p35S::CsLOX2.6 and p35S::CsLOX2.14) infested with P. rapae larvae were more attractive to C. glomerata than wild type (wt) and produced more jasmonic acid. p35S::CsLOX2.14 had a higher incidence of parasitism of the larvae than did wt. Infested transgenic plants emitted more (Z)-3-hexenyl acetate, (E)--ocimene, (Z)--ocimene, and DMNT than infested wt plants; (Z)-3-hexenyl acetate is known to attract Cotesia wasps. Transforming cruciferous crops with cytosolic CsLOX2 could aid integrated pest management via tritrophic interactions.