CULTURED HUMAN LANGERHANS CELLS RESEMBLE LYMPHOID DENDRITIC CELLS IN PHENOTYPE AND FUNCTION
CULTURED HUMAN LANGERHANS CELLS RESEMBLE LYMPHOID DENDRITIC CELLS IN PHENOTYPE AND FUNCTION
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DOI：
10.1111/1523-1747.ep12319727
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发表时间：
1989-11-01
影响因子：
6.5
通讯作者：
SCHULER, G
中科院分区：
文献类型：
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作者：
ROMANI, N;LENZ, A;SCHULER, G
新鲜分离的鼠表皮Langerhans细胞（LC）是静息T细胞的弱刺激剂。培养其表型变化后，它们的刺激活性显着增加，并且它们类似于淋巴样细胞。因此，常驻鼠LC可能代表未成熟树突状细胞的储层。现在，我们已经使用了细胞化学酶，大约80个单克隆抗体的面板，免疫荧光显微镜或两色流式细胞仪以及透射电子显微镜，以分析人类LC前后2-4 d bulk 2-4 d的表型和形态。表皮细胞培养。此外，LC从大量表皮细胞培养物中富集，并在同种异体混合白细胞反应和氧化有丝分裂发生分析中测试其刺激能力。培养的人LC类似于形态，表型和功能中的人淋巴树突状细胞。具体而言，LC对培养和开发的类似薄片的过程（所谓的“面纱”）变得不遵守，减少了其表面ATP/ADP'ASE活性，并且失去了非特异性酯酶活性。与小鼠一样，MHC I类和II类抗原的表面表达显着增加，FCII受体显着降低。在培养后LC上出现在LC上，由树突状细胞表达的标记（如CD40）。培养的人LC是有效的T细胞刺激剂。我们的发现支持这样一种观点，即像鼠LC这样的常驻人LC代表皮肤淋巴淋巴结中淋巴样细胞的未成熟前体。
Freshly isolated murine epidermal Langerhans cells (LC) are weak stimulators of resting T cells. Upon culture their phenotype changes, their stimulatory activity increases significantly, and they come to resemble lymphoid dendritic cells. Resident murine LC, therefore, might represent a reservoir of immature dendritic cells. We have now used enzyme cytochemistry, a panel of some 80 monoclonal antibodies, and immunofluorescence microscopy or two-color flow cytometry, as well as transmission electron microscopy, to analyse the phenotype and morphology of human LC before and after 2-4 d of bulk epidermal cell culture. In addition, LC were enriched from bulk epidermal cell cultures, and their stimulatory capacity was tested in the allogeneic mixed leukocyte reaction and the oxidative mitogenesis assay. Cultured human LC resembled human lymphoid dendritic cells in morphology, phenotype, and function. Specifically, LC became non-adherent upon culture and developed sheet-like processes (so-called "veils"), decreased their surface ATP/ADP''ase activity, and lost nonspecific esterase activity. As in the mouse, surface expression of MHC class I and II antigens increased significantly, and FcII receptors were significantly reduced. Markers that are expressed by dendritic cells (like CD40) appeared on LC following culture. Cultured human LC were potent T-cell stimulators. Our findings support the view that resident human LC, like murine LC, represent immature precursors of lymphoid dendritic cells in skin-draining lymph nodes.