Nucleotide sequence and restriction fragment length polymorphism analysis of the long terminal repeat of human T cell leukemia virus type II.
Nucleotide sequence and restriction fragment length polymorphism analysis of the long terminal repeat of human T cell leukemia virus type II.
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人T细胞白血病病毒II型长末端重复序列的核苷酸序列和限制性片段长度多态性分析。
DOI：
10.1089/aid.1995.11.625
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Hall,WW
中科院分区：
文献类型：
--
作者：
Eiraku,N;Monken,C;Kubo,T;Zhu,SW;Rios,M;Bianco,C;Hjelle,B;Nagashima,K;Hall,WW
分子生物学研究表明，人类T细胞白血病病毒II型存在两种主要亚型：HTLV-IIa和HTLV-IIb。为了进一步对该病毒家族进行分类，我们对长末端重复序列（LTR）进行了核苷酸序列和限制性片段长度多态性（RFLP）分析，该区域在以前的研究中已被证明具有最大的亚型内和亚型间基因组差异。核苷酸序列的分析表明，存在不同的系统发育组在每个亚型，限制性内切酶位点的预测差异的基础上，RFLP分析允许在IIa亚型（a1-a4）和IIb亚型（b1-b6）内的6个组的鉴定。核苷酸序列分析也表明可能存在HTLV-II准种。然而，这似乎并不重要，初步研究表明，这些不会影响RFLP分析的结果明显。从纽约市血液中心的HTLV-II血清阳性献血者中随机选择36个样本进行分析，证明了RFLP方法的有效性，其中可以表明所有样本都可以成功分类。此外，RFLP分析正确地匹配了四种情况下，其中HTLV-II是无意中通过输血传播的受污染血液的供体和受体中的病毒。LTR的RFLP分析似乎是鉴定HTLV-II感染的快速可靠的方法。这将证明对流行病学研究和对非土著和土著人口中存在的病毒的定性是有用的。
Molecular studies have demonstrated the existence of two major subtypes of human T cell leukemia virus type II: HTLV-IIa and HTLV-IIb. In attempts to further classify this family of viruses we have carried out nucleotide sequence and restriction fragment length polymorphism (RFLP) analysis of the long terminal repeat (LTR), a region that has been shown in previous studies to have the greatest intra- and intersubtype genomic divergence. Analysis of the nucleotide sequences suggested the existence of distinct phylogenetic groups in each subtype and, on the basis of predicted differences in restriction endonuclease sites, RFLP analysis allowed the identification of four groups within the IIa subtype (a1-a4) and six within the IIb subtype (b1-b6). Nucleotide sequence analysis also suggested the possible existence of HTLV-II quasispecies. However, this appeared not to be significant, and preliminary studies suggest that these would not be expected to influence the results of RFLP analysis appreciably. The validity of the RFLP method was demonstrated in an analysis of 36 randomly chosen samples from HTLV-II seropositive blood donors from the New York City Blood Center, where it could be shown that all could be successfully classified. Moreover, the RFLP analysis correctly matched the viruses in donors and recipients of contaminated blood in four situations in which HTLV-II was inadvertently transmitted by transfusion. RFLP analysis of the LTR appears to be a rapid and reliable method by which to identify HTLV-II infection. This should prove useful in studies of the epidemiology and the characterization of viruses present both in nonindigenous and indigenous populations.