Stromal interaction molecule 1 plays an important role in gastric cancer progression.
Stromal interaction molecule 1 plays an important role in gastric cancer progression.
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DOI：
10.3892/or.2016.4704
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发表时间：
2016-06
期刊：
影响因子：
4.2
通讯作者：
Jinmei Xu;Yan Zhou;Long Gao;Shu-xian Zhou;Weihua Liu;Xiao-an Li
中科院分区：
文献类型：
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作者：
Jinmei Xu;Yan Zhou;Long Gao;Shu-xian Zhou;Weihua Liu;Xiao-an Li
研究表明，间质相互作用分子1(STIM1)在多种癌症中都有表达，并与肿瘤生长有关。本研究旨在探讨STIM1在胃癌中的表达及其作用。免疫组织化学和Western blotting显示STIM1在胃癌组织中的表达(82%)高于正常胃组织(42%)。此外，STIM1在肿瘤血管内皮细胞中也有表达。分别用四甲基偶氮唑盐比色法、流式细胞仪、细胞黏附法和Transwell法检测STIM1对胃癌细胞增殖、凋亡、黏附、侵袭和迁移的影响。结果表明，STIM1基因敲除不改变胃癌细胞的增殖和凋亡，但促进细胞黏附，抑制细胞的迁移和侵袭。此外，STIM1基因敲除并没有改变丝裂原活化蛋白激酶(MEK)或细胞外信号调节激酶(ERK)的表达或磷酸化，这意味着STIM1通过一条独立于MEK/ERK通路的途径影响胃癌细胞的迁移。
Studies have shown that stromal interaction molecule 1 (STIM1) is expressed in a variety of cancers and is related to tumor growth. The present study aimed to investigate the expression and roles of STIM1 in gastric carcinoma. Immunohistochemistry and western blotting revealed that STIM1 was expressed at higher levels in gastric cancer tissues (82%) than these levels in normal gastric tissues (42%). In addition, STIM1 was also expressed in tumor vascular endothelial cells. The effects of STIM1 on proliferation, apoptosis, adhesion, invasion and migration of gastric cancer cells were detected by MTT assay, flow cytometry, cell adhesion assay and Transwell assay, respectively. The results shown that STIM1 knockdown did not alter proliferation or apoptosis, but promoted cell adhesion and inhibited migration and invasion in the gastric cancer cells. In addition, STIM1 knockdown did not alter the expression or phosphorylation of mitogen-activated protein kinase (MEK) or extracellular signal-regulated kinase (ERK), implying that STIM1 affected gastric cancer cell migration through a pathway independent of the MEK/ERK pathway.