REJECTION OF MARROW ALLOGRAFTS: IMPORTANCE OF H‐2 HOMOZYGOSITY OF DONOR CELLS
REJECTION OF MARROW ALLOGRAFTS: IMPORTANCE OF H‐2 HOMOZYGOSITY OF DONOR CELLS
复制标题
同种异体骨髓移植的排斥：供体细胞 H-2 纯合性的重要性
作者：
M. Bennett
C57 BL和DBA/2，但不是C57 BL X DBA/2，骨髓细胞被非免疫致死照射的C3 H X C57 BL小鼠排斥。（C57 BL X DBA/2）F1 X DBA/2或X C57 BL回交小鼠分别进行H-2和C3 H X C57 BL宿主中骨髓细胞生长的分型。每个H-2b/H-2d移植物增殖，而每个H-2b/H-2b或H-2d/H-2d移植物不能生长。因此，供体细胞H-2纯合性是骨髓移植排斥反应的先决条件之一。各种近交系或F1杂交骨髓细胞注入到一系列的辐射主机，移植的成功或失败不能与已知的血清学检测的H-2同种抗原的纯合性。因此，造血组织相容性（Hh）基因在H-2区决定抗原相关的辐射主机排斥反应。WB X DBA/2、WB X B10.D2和WB X I仅为H-2杂合细胞。DBA/2、WB X DBA/2和WB X B10.D2细胞在C57 BL X DBA/2宿主中生长，但被WB X C57 BL小鼠排斥，即使供体对于Hh特异性是纯合的，并且两种类型的受体对于相同的特异性是杂合的。Hh纯合（亲本）骨髓细胞的杂合子（F1杂种）的接受或排斥的机制，提出了需要存在一个紧密相连的“开关”基因控制表达的Hh特异性的顺式位置。
C57BL and DBA/2, but not C57BL X DBA/2, marrow cells were rejected by non-immunized lethally irradiated C3H X C57BL mice. (C57BL X DBA/2)F1 X DBA/2 or X C57BL backcross mice were individually typed for H-2 and for growth of marrow cells in C3H X C57BL hosts. Each H-2b/H-2d graft proliferated while each H-2b/H-2b or H-2d/H-2d graft failed to grow. Thus, H-2 homozygosity of donor cells is one prerequisite for marrow allograft rejection. Various inbred strain or F1 hybrid marrow cells were infused into a series of irradiated hosts; success or failure of the grafts could not be correlated with homozygosity for known serologically detectable H-2 alloantigens. Thus, Hemopoietic histocompatibility (Hh) genes at the H-2 region determine antigens relevant for rejection by irradiated hosts. WB X DBA/2, WB X B10.D2, and WB X I were only H-2 heterozygous cells rejected. DBA/2, WB X DBA/2, and WB X B10.D2 cells grew in C57BL X DBA/2 hosts but were rejected by WB X C57BL mice, even though the donors were homozygous for a Hh specificity and both types of recipients were heterozygous for that same specificity. A mechanism for acceptance or rejection of Hh homozygous (parental) marrow cells by heterozygotes (F1 hybrids) is proposed which requires the existence of a closely linked “switch‘’ gene controlling expressivity of Hh specificities in the cis position only.