Phospholipid imaging of zebrafish exposed to fipronil using atmospheric pressure matrix-assisted laser desorption ionization mass spectrometry
Phospholipid imaging of zebrafish exposed to fipronil using atmospheric pressure matrix-assisted laser desorption ionization mass spectrometry
复制标题
使用大气压基质辅助激光解吸电离质谱法对暴露于氟虫腈的斑马鱼进行磷脂成像
DOI：
10.1016/j.talanta.2019.120357
复制
发表时间：
2020-03-01
期刊：
影响因子：
6.1
通讯作者：
Liu, Huwei
中科院分区：
文献类型：
--
作者：
Liu, Wenjie;Nie, Hongxiang;Liu, Huwei
质谱成像能有效地检测和反映分子空间分布的信息，已广泛应用于生物体内内源或外源分子的原位分析。本文应用大气压基质辅助激光解吸电离质谱（AP-MALDI-MS）对氟虫腈染毒的斑马鱼全脑切片进行了成像研究。在m/z 600-950范围内的化学指纹图谱显示，暴露于氟虫腈的斑马鱼组与未处理的斑马鱼组之间的磷脂存在显著差异。结果表明，氟虫腈对斑马鱼的磷脂代谢有明显的影响，尤其是鱼眼区的磷脂代谢。本工作为农药在动物体内的毒理学研究及相关代谢分析提供了一种新的方法或可能。
Mass spectrometry imaging can effectively detect and reflect the information of molecular spatial distribution, and has been widely used for in situ analysis of endogenous or exogenous molecules in organisms. The present work applied the atmospheric pressure matrix-assisted laser desorption ionization mass spectrometry (AP-MALDI-MS) in the imaging of whole zebrafish slices exposed to fipronil. The chemical fingerprints in the range of m/z 600-950 showed significant differences in phospholipids between the fipronil exposed and untreated zebrafish groups. The major perturbed phospholipids were identified as PC(34:2), PC(34:1), PC(34:2)+Na, PC(36:4), PC(38:6), and PS(18:0/22:6), PI(18:0/20:4), PI(18:1/20:4) etc. Our results indicated that the exposure of fipronil obviously affected the phospholipid metabolism of zebrafish, especially of the fish eye region. Our work provides a new method or possibility for toxicological study and related metabolic analysis of pesticides in animals.