Surface modification of silicon and gold-patterned silicon surfaces for improved biocompatibility and cell patterning selectivity
Surface modification of silicon and gold-patterned silicon surfaces for improved biocompatibility and cell patterning selectivity
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DOI：
10.1016/j.bios.2004.06.025
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发表时间：
2005-03-15
影响因子：
12.6
通讯作者：
Zhang, MQ
中科院分区：
文献类型：
--
作者：
Lan, S;Veiseh, M;Zhang, MQ
通过自组装技术对纯净硅和金图案硅平台进行了甲氧基聚乙二醇改性，显著提高了它们的抗血浆蛋白能力和细胞构图选择性。以纤维蛋白原和免疫球蛋白为模型血浆蛋白，研究聚乙二醇层对蛋白质吸附的抑制作用。用巨噬细胞和成纤维细胞研究了金图案硅衬底上金区域的选择性细胞图案和组织相容性。这项研究还揭示了硅衬底上金电极的存在如何影响电池图案化的选择性。实验结果表明，聚乙二醇改性的硅表面对蛋白质和细胞的附着具有较高的耐受性，聚乙二醇改性的金图案硅表面几乎完全消除了蛋白质在硅表面的吸附和细胞的附着。这项研究提供了一种新的方法来开发基于硅基生物MEMS器件的生物兼容表面，特别是对于必须强制执行金属绝缘体格式的生物传感器。(C)2004爱思唯尔B.V.保留所有权利。
Clean silicon and gold-patterned silicon platforms were modified with methoxy-polyethylene glycol (M-PEG silane) via a self-assembly technique, which significantly improved their plasma protein resistance capability and cell patterning selectivity. Fibrinogen and IgG were used as model plasma proteins to study the efficacy of PEG layers in resisting protein adsorption. Selective cell patterning on the gold regions of a gold-patterned silicon substrate and tissue compatibility were studied with macrophage and fibroblast cells. The research also revealed how the presence of gold electrodes on a silicon substrate would influence the cell patterning selectivity. Our experimental results showed that the PEG-modified silicon surfaces had a high resistivity to protein and cell attachment and that the PEG-modified gold-patterned silicon surfaces nearly completely eliminated the protein adsorption and cell attachment on silicon. This study provides a new approach to developing biocompatible surfaces for silicon-based BioMEMS devices, particularly for biosensors where a metal-insulator format must be enforced. (C) 2004 Elsevier B.V. All rights reserved.