CHEMOTAXIS TOWARD SUGARS IN ESCHERICHIA-COLI
CHEMOTAXIS TOWARD SUGARS IN ESCHERICHIA-COLI
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DOI：
10.1128/jb.115.3.824-847.1973
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发表时间：
1973-01-01
影响因子：
3.2
通讯作者：
DAHL, MM
中科院分区：
文献类型：
--
作者：
ADLER, J;HAZELBAUER, GL;DAHL, MM
使用定量测定趋化性的方法，我们测试了各种糖和糖衍生物吸引大肠杆菌的能力。最有效的引诱剂，即阈值在10−5M或以下的引诱剂，非-乙酰-d-葡萄糖胺、6-脱氧-d-葡萄糖、d-果糖、d-岩藻糖、1-d-甘油-β-d-半乳糖苷、半乳糖、d-半乳糖、d-氨基葡萄糖、d-葡萄糖、α-d-葡萄糖-1-磷酸、乳糖、麦芽糖、d-甘露醇、d-甘露糖、d-甘露糖、甲基-β-d-半乳糖苷、甲基-β-d-葡萄糖苷、d-核糖、d-山梨醇和海藻糖。乳糖和可能的葡萄糖-1-磷酸只有在转化为游离单糖后才具有吸引力，而其他引诱剂不需要分解以获得趋同性。九种不同的化学感受器参与检测这些不同的引诱剂。它们被称为乙酰氨基葡萄糖、果糖、半乳糖、葡萄糖、麦芽糖、甘露醇、核糖、山梨醇和海藻糖化学受体；对每种受体的特异性进行了研究。化学感受器，除了一个福特-葡萄糖，是可以诱导的。半乳糖结合蛋白是半乳糖化学受体的识别成分。大肠杆菌对麦芽糖和d-核糖也具有渗透电击结合活性，而麦芽糖和d-核糖似乎是相应化学受体的识别成分。
Using a quantitative assay for measuring chemotaxis, we tested a variety of sugars and sugar derivatives for their ability to attractEscherichia colibacteria. The most effective attractants, i.e., those that have thresholds near 10−5M or below, areN-acetyl-d-glucosamine, 6-deoxy-d-glucose,d-fructose,d-fucose, 1-d-glycerol-β-d-galactoside, galactitol,d-galactose,d-glucosamine,d-glucose, α-d-glucose-1-phosphate, lactose, maltose,d-mannitol,d-mannose, methyl-β-d-galactoside, methyl-β-d-glucoside,d-ribose,d-sorbitol, and trehalose. Lactose, and probablyd-glucose-1-phosphate, are attractive only after conversion to the free monosaccharide, while the other attractants do not require breakdown for taxis. Nine different chemoreceptors are involved in detecting these various attractants. They are called theN-acetyl-glucosamine, fructose, galactose, glucose, maltose, mannitol, ribose, sorbitol, and trehalose chemoreceptors; the specificity of each was studied. The chemoreceptors, with the exception of the one ford-glucose, are inducible. The galactose-binding protein serves as the recognition component of the galactose chemoreceptor.E. colialso has osmotically shockable binding activities for maltose andd-ribose, and these appear to serve as the recognition components for the corresponding chemoreceptors.