lac Promoter mutations located downstream from the transcription start site.
lac Promoter mutations located downstream from the transcription start site.
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lac 启动子突变位于转录起始位点下游。
DOI：
10.1016/0022-2836(80)90145-x
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发表时间：
1980
影响因子：
5.6
通讯作者：
Reznikoff,WS
中科院分区：
文献类型：
--
作者：
Maquat,LE;Thornton,K;Reznikoff,WS
有助于定义启动子和操纵子之间重叠程度的 lac 启动子突变已被分离和表征。lacPr115 部分缓解了 lac 表达对分解代谢基因激活蛋白的依赖性。Pr115 是碱基对 +1（体外转录起始位点）处的 A/T → T/A 颠换，对阻遏子-操纵子相互作用没有影响。S7 是由 L. Johnsrud 和 J. Miller 分离和测序的突变（个人通信）位于碱基对+6。它表现出不依赖于蛋白质的部分分解代谢物基因激活剂表型和 Oc 表型。Pr111 和 Pr112 是碱基对 +10 处的 A/T 颠换。Pr111 和 Pr112 还产生不依赖于蛋白质的部分分解代谢物基因激活剂和 Oc 表型。因此，转录起始位点及其下游的序列在确定功能性RNA聚合酶-启动子相互作用的速率中发挥着作用。
lacpromoter mutations which help to define the degree of overlap between the promoter and operator have been isolated and characterized.lacPr115 partially relieves the dependence oflacexpression on the catabolite gene activator protein.Pr115 is an A/T → T/A transversion at base-pair +1 (the site of transcription initiationin vitro) that has no effect on the repressor-operator interaction.S7is a mutation isolated and sequenced by L. Johnsrud & J. Miller (personal communication) located at base-pair +6. It exhibits a partial catabolite gene activator protein-independent phenotype and an Ocphenotype.Pr111 and Pr112 are A/T transversions at base-pair +10.Pr111 andPr112 also generate a partial catabolite gene activator protein-independent and Ocphenotype. Thus, sequences at and downstream from the transcription start site play a role in determining the rate of a functional RNA polymerase-promoter interaction.