Transcriptome Analysis of White-Rot Fungi in Response to Lignocellulose or Lignocellulose-Derived Material Using RNA Sequencing Technology
Transcriptome Analysis of White-Rot Fungi in Response to Lignocellulose or Lignocellulose-Derived Material Using RNA Sequencing Technology
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DOI：
10.4236/abb.2020.118025
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发表时间：
2020-08
期刊：
影响因子：
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通讯作者：
Yixing Zhang;Koichi Yamaura
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文献类型：
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作者：
Yixing Zhang;Koichi Yamaura
白腐真菌是唯一能够完全降解木质纤维素生物质的所有组分的生物体，包括柠檬酸木质素聚合物。木质素降解对于木质纤维素生物质作为生产增值化学品和材料的原料的工业应用是重要的。因此，阐明白腐真菌中木质素的降解机制将有助于研究人员开发高效和生态友好的方法，从而能够从木质纤维素生物质中生产增值化学品。转录组分析是在不同条件下比较不同样品之间的基因表达模式的有效方法，并且可以提供对生物过程的见解。下一代测序技术，特别是RNA测序的民主化，使转录组测序和分析成为许多实验室的常用研究方法。在这篇综述中，我们专注于两个良好的特点白腐真菌（黄孢原毛革菌和角叉藻）在响应各种木质纤维素材料的转录谱。RNA-seq技术与其他技术相结合的应用仍然是研究真菌分泌组和阐明真菌对木质纤维素反应机制的最佳方法。
White-rot fungi are the only organisms that can completely degrade all components of lignocellulosic biomass, including the recalcitrant lignin polymer. Lignin degradation is important for the industrial application of lignocellulosic biomass as a raw material for producing value-added chemicals and materials. Therefore, elucidating the lignin degradation mechanism in white-rot fungi will help researchers develop efficient and eco-friendly methods enabling the production of value-added chemicals from lignocellulosic biomass. A transcriptome analysis is an effective way to compare gene expression patterns between different samples under diverse conditions and can provide insights into biological processes. The democratization of next-generation sequencing technology, especially RNA-sequencing, has made transcriptome sequencing and analysis a common research approach for many laboratories. In this review, we focus on the transcriptome profiles of two well-characterized white-rot fungi (Phanerochaete chrysosporium and Dichomitus squalens) in response to various lignocellulosic materials. The application of RNA-seq technology combining with other techniques remains the best approach for investigating fungal secretomes and elucidating the mechanisms of fungal responses to lignocellulose.