Identification of 5-Fluoro ADB in Human Whole Blood in Four Death Cases
Identification of 5-Fluoro ADB in Human Whole Blood in Four Death Cases
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DOI：
10.1093/jat/bkx088
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发表时间：
2018-03-01
影响因子：
2.5
通讯作者：
Funayama, Masato
中科院分区：
文献类型：
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作者：
Usui, Kiyotaka;Fujita, Yuji;Funayama, Masato
日本仙台在3周内发生了4起疑似由特制药物引起的死亡事件。在每一起案件中，死者都有同样的香包，标有“红心黑”，里面有一种带有芳香的干燥植物材料。我们的分析显示，该产品含有合成的大麻素受体激动剂5-氟-ADB(甲基2-[1-(5-fluoropentyl)-1H-indazole-3-carboxamide]-3，3-dimethylbutanoate，，又称5-氟-MDMB-PINACA)，在造成多人伤亡后，现已被日本列为限量设计药物。对于标准样品，全血中5-氟-ADB的测定范围为0.04~4 ng/mL，加标回收率为94.6~98.1%，检出限为6pg/mL，定量限为40pg/mL。日内精密度为0.9-4.8%，日间精密度为1.1-6.6%。偏差为-1.1%~2.9%。我们能够确认5-氟ADB在所有四名死者的血液中的浓度为0.11-1.92 ng/mL。从尸检、毒理学结果和病例周围情况来看，吸入5-氟-ADB可能是导致死亡的原因之一。然而，由于目前缺乏关于该化合物的毒理学信息，5-氟ADB在多大程度上导致了死亡仍不清楚。在未来的研究中，5-氟ADB对人体的毒性以及这种作用的机制还需要阐明。
Four deaths that seemed to have been caused by a designer drug occurred within a 3-week period in Sendai, Japan. In each case, the decedent possessed the same sachet, labeled "Heart Shot BLACK", which contained a dried plant material with an aromatic scent. It was revealed in our analysis that the product contained a synthetic cannabinoid receptor agonist, 5-fluoro ADB (methyl 2-[1-(5-fluoropentyl)-1H-indazole-3-carboxamide]-3,3-dimethylbutanoate, also known as 5-fluoro MDMB-PINACA), which is now classified as a restricted designer drug in Japan after it caused several casualties. For standard samples, the detection of 5-fluoro ADB in whole blood in the calibration range (0.04-4 ng/mL) was successful with recoveries of 94.6-98.1%, limits of detection of 6 pg/mL, and limits of quantification of 40 pg/mL. The intraday and interday precisions were 0.9-4.8% and 1.1-6.6%, respectively. The bias was -1.1 to 2.9%. We were able to confirm that 5-fluoro ADB was present in the blood of all four decedents at a concentration of 0.11-1.92 ng/mL. From the autopsy, toxicological findings, and circumstances surrounding the cases, it was considered that inhalation of 5-fluoro ADB could have contributed to the deaths. However, the extent to which 5-fluoro ADB contributed to the deaths remains unclear due to the current lack of toxicological information on the compound. In future research, the toxicity of 5-fluoro ADB in humans and the mechanism underlying this effect need to be elucidated.