Aging Is Associated With Impaired Postprandial Response of Skeletal Muscle Protein Synthesis to High-Intensity Muscle Contraction in Mice
Aging Is Associated With Impaired Postprandial Response of Skeletal Muscle Protein Synthesis to High-Intensity Muscle Contraction in Mice
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DOI：
10.1093/gerona/glad014
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发表时间：
2023-01-12
影响因子：
5.1
通讯作者：
Ogasawara，Riki
中科院分区：
文献类型：
--
作者：
Ato，Satoru;Matsunami，Hiroshi;Ogasawara，Riki
本研究的目的是调查衰老是否会改变营养状况对收缩引起的肌肉蛋白质代谢的影响。在过夜禁食或进食状态下，通过经皮电刺激使年轻（3个月）和老年（24个月）雄性C57BL/6J小鼠的右侧腓肠肌等长收缩。左侧腓肠肌作为对照。在禁食状态下，年轻和年老小鼠的基础或收缩诱导的肌肉蛋白合成没有差异。然而，在进食状态下，年轻小鼠的基础肌肉蛋白质合成较多，而收缩仅增加年轻小鼠的肌肉蛋白质合成。在进食状态下，尽管 4E-BP1 的磷酸化在两个年龄段的收缩中都有相似的增加，但 p70S6K 磷酸化的增加在年轻小鼠中更大。我们的研究结果表明，衰老会损害整合肌肉收缩和营养信号的能力，导致衰老引起的餐后状态下肌肉收缩的合成代谢阻力。
The purpose of this study was to investigate whether aging alters the effect of nutritional status on contraction-induced muscle protein metabolism. In an overnight fasted or fed states, the right gastrocnemius muscle of young (3 months) and aged (24 months) male C57BL/6J mice was isometrically contracted via percutaneous electrical stimulation. The left gastrocnemius muscle served as a control. In the fasted state, there were no differences in basal or contraction-induced muscle protein synthesis between young and old mice. However, in the fed state, basal muscle protein synthesis was greater in young mice, and contraction increased muscle protein synthesis only in young mice. In the fed state, although phosphorylation of 4E-BP1 was similarly increased by contraction in both ages, the increase in phosphorylation of p70S6K was greater in young mice. Our results indicate that aging impairs the ability to integrate signals from muscle contraction and nutrition, leading to aging-induced anabolic resistance to muscle contraction in the postprandial state.