Adenovirus E1A specifically blocks SWI/SNF-dependent transcriptional activation.
Adenovirus E1A specifically blocks SWI/SNF-dependent transcriptional activation.
复制标题
腺病毒 E1A 特异性阻断 SWI/SNF 依赖性转录激活。
DOI：
10.1128/mcb.16.10.5737
复制
发表时间：
1996
影响因子：
5.3
通讯作者：
Smith,MM
中科院分区：
文献类型：
--
作者：
Miller,ME;Cairns,BR;Levinson,RS;Yamamoto,KR;Engel,DA;Smith,MM
Expression of the adenovirus E1A243oncoprotein inSaccharomyces cerevisiaeproduces a slow-growth phenotype with accumulation of cells in the G, phase of the cell cycle. This effect is due to the N-terminal and CR1 domains of E1A243, which in rodent cells are involved in triggering cellular transformation and also in binding to the cellular transcriptional coactivator p300. A genetic screen was undertaken to identify genes required for the function of E1A243inS. cerevisiae.This screen identifiedSNF12, a gene encoding the 73-kDa subunit of the SWI/SNF transcriptional regulatory complex. Mutation of genes encoding known members of the SWI/SNF complex also led to loss of E1A function, suggesting that the SWI/SNF complex is a target of E1A243. Moreover, expression of E1A in wild-type cells specifically blocked transcriptional activation of theINO1andSUC2genes, whose activation pathways are distinct but have a common requirement for the SWI/SNF complex. These data demonstrate a specific functional interaction between E1A and the SWI/SNF complex and suggest that a similar interaction takes place in rodent and human cells.
