Kit-independent mast cell adhesion mediated by Notch
Kit-independent mast cell adhesion mediated by Notch
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Notch 介导的不依赖试剂盒的肥大细胞粘附
DOI：
10.1093/intimm/dxy067
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发表时间：
2019
影响因子：
4.4
通讯作者：
Shin-Ichi Hayashi
中科院分区：
文献类型：
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作者：
Akihiko Murata;Mari Hikosaka;Miya Yoshino;Lan Zhou;Shin-Ichi Hayashi
Kit/CD 117在哺乳动物肥大细胞（MC）的细胞-细胞和细胞-基质粘附中起着至关重要的作用;然而，尚不清楚其他粘附分子是否在MC的粘附中发挥重要作用。在本研究中，我们展示了一种新的非Kit依赖的小鼠培养的MCs的Notch家族成员介导的粘附机制。在转导有每个Notch配体基因的基质细胞上，Kit及其信号传导对于MC从系留到扩散的整个粘附过程变得至关重要。Notch介导的粘附MC的扩散涉及通过磷脂酰肌醇3-激酶和促分裂原活化蛋白激酶激活信号传导，类似于Kit介导的扩散。尽管激活了相同的信号通路，但Kit支持MC的粘附和存活，而Notch仅支持粘附。因此，Notch家族成员是MC的特异性粘附分子，其有效地取代Kit的粘附功能，以支持MC与周围细胞微环境的相互作用。
Kit/CD117 plays a crucial role in the cell–cell and cell–matrix adhesion of mammalian mast cells (MCs); however, it is unclear whether other adhesion molecule(s) perform important roles in the adhesion of MCs. In the present study, we show a novel Kit-independent adhesion mechanism of mouse cultured MCs mediated by Notch family members. On stromal cells transduced with each Notch ligand gene, Kit and its signaling become dispensable for the entire adhesion process of MCs from tethering to spreading. The Notch-mediated spreading of adherent MCs involves the activation of signaling via phosphatidylinositol 3-kinases and mitogen-activated protein kinases, similar to Kit-mediated spreading. Despite the activation of the same signaling pathways, while Kit supports the adhesion and survival of MCs, Notch only supports adhesion. Thus, Notch family members are specialized adhesion molecules for MCs that effectively replace the adhesion function of Kit in order to support the interaction of MCs with the surrounding cellular microenvironments.