[Inhibitory effect of arbutin on melanogenesis--biochemical study using cultured B16 melanoma cells].
[Inhibitory effect of arbutin on melanogenesis--biochemical study using cultured B16 melanoma cells].
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熊果苷对黑色素生成的抑制作用--培养B16黑色素瘤细胞的生化研究
DOI：
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
M. Fukuda
中科院分区：
文献类型：
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作者：
S. Akiu;Yumiko Suzuki;Tomohisa Asahara;Y. Fujinuma;M. Fukuda
以培养的B16黑色素瘤细胞为研究对象，研究了熊果苷(氢醌-β-D-吡喃葡萄糖苷)对黑素合成的抑制作用。熊果苷对细胞生长无抑制作用的最大浓度为5×10~(-5)M，单位细胞黑素含量较未处理细胞显著降低约39%。熊果苷处理的细胞酪氨酸酶活性显著降低。当熊果苷加入到B16黑色素瘤细胞悬液中时，熊果苷不能被水解以释放对苯二酚。此外，熊果苷可抑制B16黑色素瘤细胞粗提物中酪氨酸酶的活性。上述结果提示，熊果苷可能通过影响酪氨酸酶的合成和活性来抑制黑素细胞的生成，而不是通过杀死黑素细胞B16黑色素瘤细胞。另外，熊果苷对黑素合成的抑制作用不是通过对苯二酚起作用。
Inhibitory effect of arbutin (hydroquinone-beta-D-glucopyranoside) on the melanogenesis was studied biochemically using cultured B16 melanoma cells. The maximum arbutin concentration lacking an inhibitory effect on cell growth was 5 X 10(-5) M. At this concentration, melanin content per cell was decreased significantly to about 39%, compared with that of arbutin untreated cells. Also, tyrosinase activity of arbutin treated cells was decreased significantly. When arbutin was added to B16 melanoma cell suspension, arbutin was not hydrolyzed to liberate hydroquinone. Further, tyrosinase activity in crude preparations from B16 melanoma cells was inhibited by arbutin. From these results, it is suggested that arbutin can inhibit the melanogenesis by affecting not only the synthesis but also the activity of tyrosinase rather than by killing melanocytes B16 melanoma cells. Also, it is suggested that hydroquinone is not responsible for the inhibitory effect of arbutin on the melanogenesis.