Construction and expression in vivo of an internally deleted mouse alpha-fetoprotein gene: presence of a transcribed Alu-like repeat within the first intervening sequence.
Construction and expression in vivo of an internally deleted mouse alpha-fetoprotein gene: presence of a transcribed Alu-like repeat within the first intervening sequence.
复制标题
内部缺失的小鼠甲胎蛋白基因的体内构建和表达：在第一个插入序列中存在转录的 Alu 样重复序列。
DOI：
10.1093/nar/10.10.3099
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发表时间：
1982
影响因子：
14.9
通讯作者：
Tilghman,SM
中科院分区：
文献类型：
--
作者：
Young,PR;Scott,RW;Hamer,DH;Tilghman,SM
将小鼠甲胎蛋白（AFP）基因的前两个外显子沿着与其最后一个外显子连接，并在其5′侧翼序列上加0.9个碱基，在3′侧翼序列上加0.4个碱基，构建了一个AFP“小基因”。通过将这种“小基因”插入SV 40载体并感染非洲绿色猴肾细胞来测试其活性。观察到正确的起始、终止、多聚腺苷酸化和剪接。此外，检测到一个220个核苷酸的转录物，并将其定位到与第一间插序列中编码AFP基因的链相对的链上的小鼠类B1或B1重复序列。
An α-fetoprotein (AFP)*‘minigene’ was constructed by joining the first two exons along with 0.9 kilobases of 5′ flanking sequence of the mouse AFP gene to its last exon and 0.4 kilobases of 3′ flanking sequence. This ‘minigene’ was tested for activity by inserting it into an SV40 vector and infecting African green monkey kidney cells. Correct initiation, termination, polyadenylation and splicing were observed. Furthermore a 220 nucleotide transcript was detected and mapped to a mouse Alu-like or Bl repeat on the opposite strand to that encoding the AFP gene in the first intervening sequence.