Characterization of protein release from poly(ethylene glycol) hydrogels with crosslink density gradients
Characterization of protein release from poly(ethylene glycol) hydrogels with crosslink density gradients
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DOI：
10.1002/jbm.a.34701
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发表时间：
2014-02-01
影响因子：
4.9
通讯作者：
Kizilel, Seda
中科院分区：
文献类型：
--
作者：
Bal, Tugba;Kepsutlu, Burcu;Kizilel, Seda
在聚乙二醇（PEG）水凝胶支架内进行细胞移植作为有效的免疫隔离屏障正成为组织工程和再生医学的重要策略。在这些应用中，这些膜的交联密度对许多生物分子如营养物、细胞废物和激素的扩散控制具有显著影响。当这些网络被设计成具有交联密度梯度时，网络结构的改变可能对生物分子扩散性产生影响。本工作的目标是通过表面引发的光聚合合成PEG水凝胶，用于涉及生理蛋白质递送和细胞包封的应用。为了这个目的，不同的交联密度梯度的PEG水凝胶形成通过表面引发的光聚合，并通过这些PEG水凝胶支架具有定义的属性观察到具有各种分子量的模型蛋白质的扩散。扩散系数为10（-7）-10（-8）cm（2）/s，蛋白质扩散时间范围为5 min至30 h。结果证实，具有交联密度梯度的合成PEG水凝胶有希望用于生物活性分子的受控释放和用于配体的共价掺入以支持细胞活力。(c)2013 Wiley Periodicals，Inc. J Biomed Mater Res Part A：102A：487-495，2014.
Transplantation of cells within poly(ethylene glycol) (PEG) hydrogel scaffolds as effective immunoisolation barriers is becoming increasingly important strategy for tissue engineering and regenerative medicine. In these applications, crosslink density of these membranes has significant effect on the control of diffusion of many biomolecules such as nutrients, cellular wastes, and hormones. When these networks are designed with crosslink density gradients, alterations in network structure may have an effect on biomolecule diffusivity. The goal of this work was to synthesize PEG hydrogels via surface initiated photopolymerization for use in applications involving physiological protein delivery and cell encapsulation. For this purpose, PEG hydrogels of differing crosslink density gradients were formed via surface initiated photopolymerization, and the diffusion of model proteins with various molecular weights were observed through these PEG hydrogel scaffolds with defined properties. Diffusion coefficients were on the order of 10(-7)-10(-8) cm(2)/s and protein diffusion time scales varied from 5 min to 30 h. The results confirm that synthetic PEG hydrogels with crosslink density gradients are promising for controlled release of bioactive molecules and for covalent incorporation of ligands to support cell viability. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 487-495, 2014.