Expression and function of the zinc finger transcription factor Sp6–9 in the spider Parasteatoda tepidariorum
Expression and function of the zinc finger transcription factor Sp6–9 in the spider Parasteatoda tepidariorum
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DOI：
10.1007/s00427-017-0595-2
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发表时间：
2017-11
影响因子：
2.4
通讯作者：
T. Königsmann;Natascha Turetzek;Matthias Pechmann;Nikola-Michael Prpic
中科院分区：
文献类型：
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作者：
T. Königsmann;Natascha Turetzek;Matthias Pechmann;Nikola-Michael Prpic
Sp 6 - 9组的锌指转录因子在所有后生动物中是进化上保守的，并且在例如，四肢形成和心脏发育。Sp 6 -9相关基因的功能已在许多脊椎动物和无脊椎动物中进行了研究，但迄今为止缺乏来自螯形动物（蜘蛛及其盟友）的数据。我们已经从普通的家蜘蛛寄生虫（Parasteatoda tepidariorum）和地窖蜘蛛Pholcus phalangioides中分离到了Sp 6 - 9的直系同源物。我们发现，在这些蜘蛛物种的Sp 6 - 9基因表达的发展中的附属物，从而表明在肢体形成保守的作用。事实上，用Sp 6 - 9 inP. tepidariorum不仅导致强壮的肢体缺陷，而且在更严重的受影响动物中导致身体节段的损失和头部缺陷。结合早期胚胎中的一个新的表达域，这些数据表明Sp 6 - 9具有双重作用。冰室在头和身体节的形成早期的作用是不知道从其他节肢动物，但在肢体形成的作用是进化高度保守。
Zinc finger transcription factors of theSp6–9group are evolutionarily conserved in all metazoans and have important functions in, e.g., limb formation and heart development. The function ofSp6–9-related genes has been studied in a number of vertebrates and invertebrates, but data from chelicerates (spiders and allies) was lacking so far. We have isolated the ortholog ofSp6–9from the common house spiderParasteatoda tepidariorumand the cellar spiderPholcus phalangioides. We show that theSp6–9gene in these spider species is expressed in the developing appendages thus suggesting a conserved role in limb formation. Indeed, RNAi withSp6–9inP. tepidariorumleads not only to strong limb defects, but also to the loss of body segments and head defects in more strongly affected animals. Together with a new expression domain in the early embryo, these data suggest thatSp6–9has a dual roleP. tepidariorum. The early role in head and body segment formation is not known from other arthropods, but the role in limb formation is evolutionarily highly conserved.