Indocyanine green matching phantom for fluorescence-guided surgery imaging system characterization and performance assessment
Indocyanine green matching phantom for fluorescence-guided surgery imaging system characterization and performance assessment
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DOI：
10.1117/1.jbo.25.5.056003
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发表时间：
2020-05-01
影响因子：
3.5
通讯作者：
Pogue, Brian W.
中科院分区：
文献类型：
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作者：
Ruiz, Alberto J.;Wu, Mindy;Pogue, Brian W.
重要性：荧光引导手术的扩大使用与被批准用于吲哚菁绿色（ICG）的设备已经导致了一系列可用的商业系统。目前迫切需要能够独立表征系统性能并允许跨系统比较。目的：这项工作的目标是扩展先前提出的荧光成像标准设计，以开发一种长期稳定的体模，该体模在光谱上匹配ICG特性，并利用3D打印技术结合组织等效材料。方法：创建了一批测试目标，以评估0.3至1000 nM范围内的ICG浓度灵敏度、低至6 mm的组织等效深度灵敏度以及USAF测试图的空间分辨率。使用一系列具有显著不同成像能力和应用的系统完成比较，包括Li-Cor（R）Odyssey、Li-Cor（R）Pearl、PerkinElmer（R）Solaris和斯特赖克（R）Spy Elite。结果：使用所有四种市售系统的ICG匹配体模成像显示出基准系统性能的能力，并允许跨系统比较。荧光测试能够评估ICG可检测浓度的差异，对于临床前和临床系统，灵敏度差异> 10倍。此外，测试成功地评估了系统在深度信号衰减率、分辨率性能和图像伪影方面的差异。制造的变化，光稳定性，和机械设计的测试表明，承诺在提供长期稳定的标准荧光imaging.Conclusions：ICG匹配幻影提供了一个重要的一步，标准化的性能表征和跨系统的比较批准使用ICG的设备。开发的混合制造平台可以将长期稳定的荧光剂与3D打印的组织等效材料结合起来。此外，体模的长期测试和制造工艺的改进对于未来作为广泛采用的荧光成像标准的实施是必要的。(C)作者。由SPIE在知识共享署名4.0未移植许可下发布。
Significance: Expanded use of fluorescence-guided surgery with devices approved for use with indocyanine green (ICG) has led to a range of commercial systems available. There is a compelling need to be able to independently characterize system performance and allow for cross-system comparisons.Aim: The goal of this work is to expand on previous proposed fluorescence imaging standard designs to develop a long-term stable phantom that spectrally matches ICG characteristics and utilizes 3D printing technology for incorporating tissue-equivalent materials.Approach: A batch of test targets was created to assess ICG concentration sensitivity in the 0.3- to 1000-nM range, tissue-equivalent depth sensitivity down to 6 mm, and spatial resolution with a USAF test chart. Comparisons were completed with a range of systems that have significantly different imaging capabilities and applications, including the Li-Cor (R) Odyssey, Li-Cor (R) Pearl, PerkinElmer (R) Solaris, and Stryker (R) Spy Elite.Results: Imaging of the ICG-matching phantoms with all four commercially available systems showed the ability to benchmark system performance and allow for cross-system comparisons. The fluorescence tests were able to assess differences in the detectable concentrations of ICG with sensitivity differences >10x for preclinical and clinical systems. Furthermore, the tests successfully assessed system differences in the depth-signal decay rate, as well as resolution performance and image artifacts. The manufacturing variations, photostability, and mechanical design of the tests showed promise in providing long-term stable standards for fluorescence imaging.Conclusions: The presented ICG-matching phantom provides a major step toward standardizing performance characterization and cross-system comparisons for devices approved for use with ICG. The developed hybrid manufacturing platform can incorporate long-term stable fluorescing agents with 3D printed tissue-equivalent material. Further, long-term testing of the phantom and refinements to the manufacturing process are necessary for future implementation as a widely adopted fluorescence imaging standard. (C) The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License.