Influence of perfusion on metabolism and matrix production by bovine articular chondrocytes in hydrogel scaffolds
Influence of perfusion on metabolism and matrix production by bovine articular chondrocytes in hydrogel scaffolds
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DOI：
10.1002/bit.20818
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发表时间：
2006-04
影响因子：
3.8
通讯作者：
Xia Xu;J. Urban;U. Tirlapur;M. Wu;Z. Cui;Z. Cui
中科院分区：
文献类型：
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作者：
Xia Xu;J. Urban;U. Tirlapur;M. Wu;Z. Cui;Z. Cui
关节软骨损伤后自我修复的能力有限。工程软骨是一种很有前途的治疗方法，可以替代或修复受损组织。工程化软骨的生长对细胞外培养环境敏感。将软骨细胞以400万/mL接种到藻酸盐珠和琼脂糖支架中，并在长达12天的时间内检查对静态和灌注培养的反应。对于两种类型的支架，软骨细胞在所有培养条件下保持其分化形态超过12天。在藻酸盐珠中，在灌注培养中比在静态条件下产生更多的糖胺聚糖（GAG）。在灌注培养中，海藻酸盐构建体中的GAG分布比在静态培养中更均匀。然而，在琼脂糖构建体中，静态培养和灌注培养之间的GAG产生没有显著差异。在灌流培养条件下，海藻酸钠对GAG的留存率高于琼脂糖。提示灌注培养的积极作用只有通过适当选择支架材料等其他因素才能实现。© 2006 Wiley Periodicals，Inc.
Articular cartilage has a limited capacity for self‐repair after damage. Engineered cartilage is a promising treatment to replace or repair damaged tissue. The growth of engineered cartilage is sensitive to the extracellular culture environment. Chondrocytes were seeded into alginate beads and agarose scaffolds at 4 millions/mL, and the response to static and perfusion culture was examined over period of up to 12 days. For both types of scaffolds, the chondrocytes kept their differentiated morphology over 12 days in all culture conditions. In alginate beads, more glycosaminoglycans (GAGs) were produced in perfusion culture than in static conditions. GAG distribution in alginate constructs was more uniform in perfusion culture than in static culture. However, in agarose constructs there was no significant difference in GAG production between static culture and perfusion culture. Under perfusion culture, the retention rate of GAG in alginate was higher than in agarsoe. It is suggested that the positive effect of perfusion culture only can be achieved by an appropriate choice of other factors such as scaffold materials. © 2006 Wiley Periodicals, Inc.