SELECTIVE GROWTH-INHIBITION OF PORPHYROMONAS-GINGIVALIS BY BESTATIN
SELECTIVE GROWTH-INHIBITION OF PORPHYROMONAS-GINGIVALIS BY BESTATIN
复制标题
DOI：
10.1111/j.1574-6968.1994.tb07221.x
复制
发表时间：
1994-10-15
影响因子：
2.1
通讯作者：
MICHAUD, J
中科院分区：
文献类型：
--
作者：
GRENIER, D;MICHAUD, J
我们实验室最近的工作表明，选定的蛋白酶/肽酶抑制剂干扰牙龈卟啉单胞菌的生长。本研究的目的是进一步研究bestatin对牙龈卟啉单胞菌生长的抑制作用。当在复合肉汤培养基中加入2.5 μ g ml（-1）的bestatin时，观察到牙龈卟啉单胞菌（11株）的完全生长抑制。在最终浓度为0.5 μ g/ml时，仍能获得50%的抑制作用。Bestatin的抑制作用是高度特异性的，因为20种不同的口腔细菌的生长，包括革兰氏阳性和革兰氏阴性以及糖分解和天冬氨酸分解细菌，即使在Bestatin浓度高达50 μ g ml（-1）时也不受影响。Bestatin对牙龈卟啉单胞菌的活力没有显著影响，这表明它具有抑菌作用而不是杀菌作用。使用其他特异性抑制剂的生长测定表明，bestatin对牙龈卟啉单胞菌生长的影响不太可能与其氨肽酶抑制剂活性有关。用亚抑制浓度的bestatin培养牙龈卟啉单胞菌没有改变细胞包膜蛋白质谱，如通过SDS-PAGE分析所确定的，但显著减少了产生的细胞外囊泡的数量。本研究表明，bestatin是一种高效的牙龈卟啉单胞菌细胞生长抑制剂。进一步的研究将表明是否应将bestatin视为控制牙龈卟啉单胞菌（成人慢性牙周炎的可疑病原体）的潜在药物。
Recent work in our laboratory indicates that selected protease/peptidase inhibitors interfere with the growth of Porphyromonas gingivalis. The aim of the present study was to further investigate the inhibitory effect of bestatin on the growth of P. gingivalis. Complete growth inhibition of P. gingivalis (11 strains) was observed when bestatin was incorporated at 2.5 mu g ml(-1) in a complex broth medium. Fifty percent inhibition was still obtained with bestatin at a final concentration of 0.5 mu g ml(-1). The inhibitory effect of bestatin was highly specific as the growth of 20 different oral bacterial species, including Gram-positive and Gram-negative as well as saccharolytic and asaccharolytic bacteria, was not affected even at bestatin concentrations up to 50 mu g ml(-1) Bestatin did not significantly affect the viability of P. gingivalis indicating that it has a bacteriostatic rather than a bactericidal effect. Growth assays using other specific inhibitors suggested that the effect of bestatin on the growth of P. gingivalis was unlikely to be related to its aminopeptidase inhibitor activity. Cultivation of P. gingivalis with a subinhibitory concentration of bestatin did not modify the cell envelope protein profile, as determined by SDS-PAGE analysis, but significantly decreased the number of extracellular vesicles produced. The present study indicated that bestatin is a highly effective inhibitor of cell growth of P. gingivalis. Additional studies will indicate whether bestatin should be considered as a potential drug in the control of P. gingivalis, a suspected pathogen in adult chronic periodontitis.