ALBA4 modulates its stage-specific interactions and specific mRNA fates during Plasmodium yoelii growth and transmission
ALBA4 modulates its stage-specific interactions and specific mRNA fates during Plasmodium yoelii growth and transmission
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DOI：
10.1111/mmi.13762
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发表时间：
2017-10-01
影响因子：
3.6
通讯作者：
Lindner, Scott E.
中科院分区：
文献类型：
--
作者：
Munoz, Elyse E.;Hart, Kevin J.;Lindner, Scott E.
疟疾寄生虫的传播发生在一个不可预测的时刻，当蚊子吸血时。因此，疟原虫进化出了为传播做准备的策略，包括预防性地抑制和保护建立感染所需的mRNA。然而，这些关键控制的机制还不清楚，包括疟原虫是否在不同阶段改变其抑制性复合物和mRNA靶点。由于大量蚊子污染样本，严重的技术限制阻碍了对这一点的理解。在这里使用约氏疟原虫，我们第一次提供了一个蛋白质复合物的蛋白质组学比较跨越无性血液，有性和子孢子阶段，沿着转录组学比较的mRNA，在这些阶段受到影响。我们发现Apicomplexan特异性ALBA 4 RNA结合蛋白的作用是调节寄生虫传播阶段的发展，并且ALBA 4与阶段特异性和阶段独立性伙伴相关联以产生相反的mRNA命运。这些努力扩大了我们的理解和能力，询问性传播和子孢子传播阶段以及它们进化以延续其感染周期的分子制剂。
Transmission of the malaria parasite occurs in an unpredictable moment, when a mosquito takes a blood meal. Plasmodium has therefore evolved strategies to prepare for transmission, including translationally repressing and protecting mRNAs needed to establish the infection. However, mechanisms underlying these critical controls are not well understood, including whether Plasmodium changes its translationally repressive complexes and mRNA targets in different stages. Efforts to understand this have been stymied by severe technical limitations due to substantial mosquito contamination of samples. Here using P. yoelii, for the first time we provide a proteomic comparison of a protein complex across asexual blood, sexual and sporozoite stages, along with a transcriptomic comparison of the mRNAs that are affected in these stages. We find that the Apicomplexan-specific ALBA4 RNA-binding protein acts to regulate development of the parasite's transmission stages, and that ALBA4 associates with both stage-specific and stage-independent partners to produce opposing mRNA fates. These efforts expand our understanding and ability to interrogate both sexual and sporozoite transmission stages and the molecular preparations they evolved to perpetuate their infectious cycle.