Fishing for Developmental Regulatory Regions: Zebrafish Tissue-Specific ATAC-seq.
Fishing for Developmental Regulatory Regions: Zebrafish Tissue-Specific ATAC-seq.
复制标题
DOI：
10.1007/978-1-0716-2847-8_19
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
基因调控网络描述了转录因子和调控DNA之间的相互作用，可以提供组织发育的系统水平视图。在这里，我们描述了一个协议的分离和审讯的组织特异性顺式调控元件在斑马鱼胚胎发育过程中使用低输入ATAC-seq。利用所描述的方法，可以鉴定小群体发育组织中调控DNA的全基因组评估，从而允许构建基因调控网络。
Gene regulatory networks describe interactions between transcription factors and regulatory DNA and can provide a systems-level view of tissue development. Here, we describe a protocol for isolation and interrogation of tissue-specific cis-regulatory elements during zebrafish embryonic development using low-input ATAC-seq. With the methods described, genome-wide assessments of regulatory DNA in small populations of developing tissues can be identified allowing the construction of gene regulatory networks.