Partitioning of nasal and pulmonary resistance changes during noninvasive plethysmography in mice
Partitioning of nasal and pulmonary resistance changes during noninvasive plethysmography in mice
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DOI：
10.1152/japplphysiol.90700.2008
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发表时间：
2008-12-01
影响因子：
3.3
通讯作者：
Parikh, Indu
中科院分区：
文献类型：
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作者：
Agrawal, Anurag;Singh, Shashi Kant;Parikh, Indu
双腔体积描记术是一种成熟的非侵入性方法，用于评估小型实验室动物的气流阻塞。它允许测量比气道阻力（sRaw），与增强暂停（Penh）不同，这是一个有意义的气道力学参数。由于sRaw是在自主呼吸的小鼠中测量的，因此该方法的局限性是不能排除鼻阻力变化。我们最近发现，小鼠并不是真正的专性鼻呼吸者，在鼻腔阻塞后，鼻呼吸小鼠可以过渡到口腔呼吸模式。我们现在表明，假设口腔阻力保持不变，通过对经鼻或经口呼吸的小鼠组进行一系列测量，可以用代数方法分离总气道阻力变化的平均鼻和肺（包括喉）成分。使用这种方法，我们表明，鼻阻力的变化包括一半或更多的总阻力变化乙酰甲胆碱的挑战。抑制气道杯状细胞的粘蛋白分泌可减弱肺气道高反应性（AHR），但不能减弱鼻气道高反应性（AHR），在鼻炎小鼠模型中鼻AHR可能与水肿有关。
Double-chamber plethysmography is a well established noninvasive method of assessing airflow obstruction in small lab animals. It allows measurement of the specific airway resistance (sRaw), which unlike enhanced pause (Penh), is a meaningful airway mechanics parameter. Since sRaw is measured in spontaneously breathing mice, a limitation of the method is the inability to exclude nasal resistance changes. We recently showed that mice are not truly obligate nasal breathers and that after nasal occlusion, nasally breathing mice can transition to an oral mode of breathing. We now show that it is experimentally possible to algebraically separate the average nasal and pulmonary (including laryngeal) components of total airway resistance change by a series of measurements made across groups of mice breathing nasally or orally, assuming that oral resistance remains constant. Using this approach, we show that nasal resistance change comprises one-half or more of the total resistance change during methacholine challenge. Inhibition of mucin secretion from airway goblet cells attenuates pulmonary but not nasal airway hyperresponsiveness (AHR), and nasal AHR in a murine model of rhinitis may be related to edema.