Multinucleation and cell dysfunction induced by amorphous silica nanoparticles in an L-02 human hepatic cell line.
Multinucleation and cell dysfunction induced by amorphous silica nanoparticles in an L-02 human hepatic cell line.
复制标题
无定形二氧化硅纳米颗粒在 L-02 人肝细胞系中诱导的多核和细胞功能障碍
DOI：
10.2147/ijn.s46732
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发表时间：
2013
影响因子：
8
通讯作者：
Sun Z
中科院分区：
文献类型：
--
作者：
Wang W;Li Y;Liu X;Jin M;Du H;Liu Y;Huang P;Zhou X;Yuan L;Sun Z
Silica nanoparticles (SNPs) are one of the most important nanomaterials, and have been widely used in a variety of fields. Therefore, their effects on human health and the environment have been addressed in a number of studies. In this work, the effects of amorphous SNPs were investigated with regard to multinucleation in L-02 human hepatic cells. Our results show that L-02 cells had an abnormally high incidence of multinucleation upon exposure to silica, that increased in a dose-dependent manner. Propidium iodide staining showed that multinucleated cells were arrested in G2/M phase of the cell cycle. Increased multinucleation in L-02 cells was associated with increased generation of cellular reactive oxygen species and mitochondrial damage on flow cytometry and confocal microscopy, which might have led to failure of cytokinesis in these cells. Further, SNPs inhibited cell growth and induced apoptosis in exposed cells. Taken together, our findings demonstrate that multinucleation in L-02 human hepatic cells might be a failure to undergo cytokinesis or cell fusion in response to SNPs, and the increase in cellular reactive oxygen species could be responsible for the apoptosis seen in both mononuclear cells and multinucleated cells.
