Effect of IS-741 on cell adhesion between human umbilical vein endothelial cells and HL-60 cells.
Effect of IS-741 on cell adhesion between human umbilical vein endothelial cells and HL-60 cells.
复制标题
IS-741对人脐静脉内皮细胞与HL-60细胞粘附的影响。
DOI：
10.1248/bpb.22.127
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发表时间：
1999
影响因子：
2
通讯作者：
M. Kato
中科院分区：
文献类型：
--
作者：
H. Shikama;S. Yotsuya;S. Satake;H. Sugi;M. Kato
IS-741 （N-[（2-乙基磺酰基氨基）-5-三氟甲基-3-吡啶基]环己anecarboxamide一水合物）对胰腺炎模型的影响先前已有报道。最近对大鼠治疗试验的病理组织学观察发现，IS-741给药组显示炎症细胞（多形核白细胞）的组织浸润程度较低。因此，我们研究了细胞粘附，这是活化的中性粒细胞浸润组织的第一步，并在体外研究了脂多糖刺激下is -741对人脐静脉内皮细胞（HUVEC）和人早幼粒细胞白血病细胞系（HL-60）细胞之间细胞粘附的影响。IS-741明显抑制HL-60细胞对HUVEC的粘附。进一步对单个细胞的研究表明，IS-741主要影响HL-60细胞。对is -741在分子水平（靶向粘附分子）的抑制作用的研究也显示，is -741对内皮白细胞粘附分子-1 （ELAM-1）、细胞间粘附分子-1 （ICAM-1）或血管细胞粘附分子-1 （VCAM-1）对HUVEC的出现没有影响，支持is -741主要作用于HL-60细胞的理论，即使在分子水平上也是如此。然而，在黏附测试系统中加入抗icam -1或抗vcam -1抗体的实验中发现了对黏附的抑制作用。因此，is -741可能会影响粘附分子，这些分子属于beta1或beta2整合素家族。
The effect of IS-741 (N-[(2-ethylsulfonylamino)-5-trifluoromethyl-3-pyridyl] cyclohexanecarboxamide monohydrate) on a model for pancreatitis has been previously reported. Recent patho-histological observations of remedial tests using rats found that the IS-741 administered group showed a low degree of tissue infiltration by inflammatory cells (polymorphonuclear leukocytes). We therefore examined cell adhesion, which is the first step in tissue infiltration by activated neutrophils, and investigated the effect of IS-741 on cell adhesion between human umbilical vein endothelial cells (HUVEC) and human promyelo-leukemia cell line (HL-60) cells during lipopolysaccharide stimulation in vitro. IS-741 significantly inhibited the adhesion of HL-60 cells to HUVEC. Further investigation of IS-741 on individual cells revealed that IS-741 mainly affected HL-60 cells. Investigation of the inhibitory effect of IS-741 at the molecular level (targeting adhesion molecules) also revealed that IS-741 had no effect on the appearance of endothelial leukocyte adhesion molecule-1 (ELAM-1), intercellular adhesion molecule-1 (ICAM-1) or vascular cell adhesion molecule-1 (VCAM-1) on HUVEC, which supports the theory that IS-741 is mainly effective on HL-60 cells, even at the molecular level. However, the inhibition of adhesion was noticed in experiments in which an anti-ICAM-1 or anti-VCAM-1 antibody was added to the adhesion test system. Therefore, IS-741 is likely to affect adhesion molecules which belong to the beta1 or beta2 integrin family.