Pathogenicity of different isolates of Vibrio harveyi in tiger prawn, Penaeus monodon
Pathogenicity of different isolates of Vibrio harveyi in tiger prawn, Penaeus monodon
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DOI：
10.1111/j.1472-765x.1996.tb01192.x
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发表时间：
1996-06-01
影响因子：
2.4
通讯作者：
Chen, SN
中科院分区：
文献类型：
--
作者：
Liu, PC;Lee, KK;Chen, SN
用活菌和胞外产物（ECP）研究了6株哈维氏弧菌对斑节对虾的致病性。使用活细菌和ECP（LD（50），4.87-8.65 × 10（4）菌落形成单位（cfu）和1.20-1.51 μ g蛋白质g（-1）体重），最初从患病的对虾中分离的生物体比最初从海水中分离的两种参考菌株更具毒性（ATCC 25919; LD（50），3.18 × 10（6）cfu和2.70 μ g蛋白质g（-1）体重）或患病的Talpestia sp.（ATCC 14126，0.418 × 10（6）cfu和2.34 μ g蛋白质g（-1）体重）。在每次细菌挑战后，从濒死对虾的血淋巴和肝胰腺中重新分离每种菌株。无论是活菌和ECP的Penaeid分离物表现出更强的蛋白水解酶（酪蛋白酶），磷脂酶和溶血活性比参考菌株。这些结果表明，哈维氏弧菌在对虾体内的致病性与非对虾的哈维氏弧菌存在差异，推测蛋白酶、磷脂酶、溶血素或外毒素可能在哈维氏弧菌对斑节对虾的致病性中起主导作用。
The pathogenicity of six Vibrio harveyi strains in tiger prawn, Penaeus monodon, was studied, using both live bacteria and extracellular products (ECP). The organisms originally isolated from diseased penaeids were more virulent using both live bacteria and ECP (LD(50), 4.87-8.65 x 10(4) colony-forming units (cfu) and 1.20-1.51 mu g protein g(-1) body weight) than the two reference strains originally isolated from either sea water (ATCC 25919; LD(50), 3.18 x 10(6) cfu and 2.70 mu g protein g(-1) body weight) or diseased Talorchestia sp. (ATCC 14126, 0.418 x 10(6) cfu and 2.34 mu g protein g(-1) body weight). Each strain was reisolated from the haemolymph and the hepatopancreas of moribund prawns following each bacterial challenge. Both the live bacteria and the ECPs of the penaeid isolates exhibited stronger proteolytic (caseinase), phospholipase and haemolytic activities than those of the reference strains. These results indicate that there are differences between penaeid and non-penaeid isolates of V, harveyi in pathogenicity and reveal that proteases, phospholipases, haemolysins or exotoxins might play leading roles in the pathogenicity of V. harveyi in the tiger prawn, Penaeus monodon.