Molecular markers of cardiac endocardial cushion development
Molecular markers of cardiac endocardial cushion development
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DOI：
10.1002/dvdy.10418
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发表时间：
2003-12-01
影响因子：
2.5
通讯作者：
Gruber, PJ
中科院分区：
文献类型：
--
作者：
Gitler, AD;Lu, MM;Gruber, PJ
内膜垫是成熟心脏瓣膜的前体。它们在环状心管内形成离散的小叶，并发展成薄而柔韧的小叶，以防止血液回流。心脏瓣膜的胚胎起源包括内皮细胞、心肌细胞和神经嵴细胞。最近，越来越多的动物模型来自突变筛选，基因失活，和转基因研究已经确定了特定的分子所需的正常发育的心脏瓣膜，和关键的分子途径开始出现。为了进一步推进这一过程，我们试图组装一套不同的分子标记物，涵盖心脏瓣膜发育的所有阶段。在这里，我们提供了一个详细的比较基因表达分析的13个内膜垫标记。我们确定了转录因子Fog 1的内膜垫表达，我们证明了在发展内膜垫中活跃的Wnt/β-连环蛋白信号传导，这表明以前没有被认识到参与心脏瓣膜形成的途径。(C)2003 Wiley-Liss，Inc.
Endocardial cushions are precursors of mature heart valves. They form within the looped heart tube as discrete swellings and develop into thin, pliable leaflets that prevent regurgitation of blood. The embryonic origins of cardiac valves include endothelial, myocardial, and neural crest cells. Recently, an increasing number of animal models derived from mutational screens, gene inactivation, and transgenic studies have identified specific molecules required for normal development of the cardiac valves, and critical molecular pathways are beginning to emerge. To further this process, we have sought to assemble a diverse set of molecular markers encompassing all stages of cardiac valve development. Here, we provide a detailed comparative gene expression analysis of thirteen endocardial cushion markers. We identify endocardial cushion expression of the transcription factor Fog1, and we demonstrate active Wnt/beta-catenin signaling in developing endocardial cushions suggesting pathways that have not been previously appreciated to participate in cardiac valve formation. (C) 2003 Wiley-Liss, Inc.