BIRTH OF LARGE CALVES THAT DEVELOPED FROM IN VITRO-DERIVED BOVINE EMBRYOS
BIRTH OF LARGE CALVES THAT DEVELOPED FROM IN VITRO-DERIVED BOVINE EMBRYOS
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DOI：
10.1016/0093-691x(95)00172-5
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发表时间：
1995-07-15
期刊：
影响因子：
2.8
通讯作者：
MURRAY, JD
中科院分区：
文献类型：
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作者：
BEHBOODI, E;ANDERSON, GB;MURRAY, JD
在通过体外成熟（IVM）和体外受精（IVF）程序生产的牛胚胎发育的小牛中观察到高出生体重。体外成熟和体外受精后，胚胎与输卵管上皮细胞体外共培养或移植到绵羊输卵管中发育至囊胚期。将囊胚转移到受体小母牛和奶牛的生殖道中，以发育至足月。比较了从体外衍生胚胎发育的小牛和从选择受体雌性的牛群中奶牛人工授精（AI）后出生的小牛的出生体重和妊娠期。妊娠期各组间无差异（P > 0.05），但IVM/IVF衍生胚胎体外共培养的犊牛出生时体型大于IVM/IVF衍生胚胎在绵羊输卵管中发育成囊胚所生的犊牛和AI所生的犊牛（P < 0.001）。难产和小牛死亡率与出生时小牛尺寸大有关。这些数据是从一项为其他目的而设计的实验中收集的，混杂变量和小样本量可能会影响观察到的出生体重差异。然而，一些小牛的极端出生体重表明，在一些IVM/IVF衍生的牛胚胎中发生异常的产前生长，并且共培养至囊胚阶段的条件可能会加剧该问题。
High birth weights were observed in calves that developed from bovine embryos produced by in vitro maturation (IVM) and in vitro fertilization (IVF) procedures. After IVM and IVF, embryos were either co-cultured in vitro with oviductal epithelial cells or transferred into the sheep oviduct for development to the blastocyst stage. Blastocysts were transferred to the reproductive tracts of recipient heifers and cows for development to term. Birth weights and gestation periods were compared between calves that developed from in vitro-derived embryos and calves born after artificial insemination (AI) of cows in the herd from which recipient females were selected. Gestation periods were not different among the groups (P > 0.05), but calves that developed from IVM/IVF-derived embryos co-cultured in vitro were larger at birth than calves born from IVM/IVF-derived embryos that developed into blastocysts in the sheep oviduct and calves born from AI (P < 0.001). Dystocia and calf mortality were associated with large calf size at birth. These data were collected from an experiment designed for other purposes, and confounding variables and small sample size could have influenced the observed differences in birth weights. Nevertheless, the extreme birth weights of some calves suggest that abnormal prenatal growth occurs in some IVM/IVF-derived bovine embryos and that conditions for co-culture to the blastocyst stage may exacerbate the problem.