Formation of geometrically complex lipid nanotube-vesicle networks of higher-order topologies
Formation of geometrically complex lipid nanotube-vesicle networks of higher-order topologies
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DOI：
10.1073/pnas.172183699
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发表时间：
2002-09-03
影响因子：
11.1
通讯作者：
Orwar, O
中科院分区：
文献类型：
--
作者：
Karlsson, M;Sott, K;Orwar, O
我们提出了一种微电融合的方法来构建具有高几何复杂性的流态脂双层网络，直到具有亏格=3的拓扑结构的完全连通网络。在网络内部，可以产生自组织的分支纳米管结构，其中交叉点自发地将自己排列成三向连接，每个纳米管之间的角度为120度。分支纳米管网络的形成似乎遵循最小弯曲能量算法，该算法解决了路径最小化问题。它还证明，材料可以通过纳米管介导的具有特定内容物的卫星囊泡的传输而被注入网络中的特定容器中。利用微电融合、自发纳米管图案形成和卫星-囊泡注射的组合，可以生产容器和纳米管的复杂网络，以用于例如纳米流体和人造细胞设计的一系列应用。此外，这种电融合方法可以将生物细胞整合到脂质纳米管-囊泡网络中。
We present a microelectrofusion method for construction of fluid-state lipid bilayer networks of high geometrical complexity up to fully connected networks with genus = 3 topology. Within networks, self-organizing branching nanotube architectures could be produced where intersections spontaneously arrange themselves into three-way junctions with an angle of 120degrees between each nanotube. Formation of branching nanotube networks appears to follow a minimum-bending energy algorithm that solves for pathway minimization. It is also demonstrated that materials can be injected into specific containers within a network by nanotube-mediated transport of satellite vesicles having defined contents. Using a combination of microelectrofusion, spontaneous nanotube pattern formation, and satellite-vesicle injection, complex networks of containers and nanotubes can be produced for a range of applications in, for example, nanofluidics and artificial cell design. In addition, this electrofusion method allows integration of biological cells into lipid nanotube-vesicle networks.