Pathogenic mechanisms of influenza A(H1N1)pdm09 infection elucidated by gene expression profiling.
Pathogenic mechanisms of influenza A(H1N1)pdm09 infection elucidated by gene expression profiling.
复制标题
通过基因表达谱阐明甲型H1N1pdm09流感感染的致病机制。
DOI：
10.1111/ped.12139
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发表时间：
2013
期刊：
影响因子：
1.4
通讯作者：
Morishima T.
中科院分区：
文献类型：
--
作者：
Yamashita N;Tsukahara H;Tsuge M;Nagaoka Y;Yashiro M;Saito Y;Fujii Y;Takashi O;Morishima T.
背景甲型H1N1流感pdm 09相关中枢神经系统（CNS）表现和肺炎的致病机制尚不清楚。本研究使用患者外周血的基因表达谱检查A（H1N1）pdm 09宿主反应。方法对三组16名A（H1N1）pdm 09感染的儿童进行了检查：CNS组，惊厥和意识改变（n= 6）;肺炎（Pneu）组（n= 5）;和一组感染的对照患者（n= 5）。CNS或Pneu的急性恢复期的信号比进行了分析与那些control.ResultsThe CNS（619成绩单）和Pneu（656成绩单）组有显着增加的信号比相比，对照组。关于多个探针显示的转录物比例增加，在CNS和Pneu中观察到接触蛋白相关蛋白样3转录物、油酰-ACP水解酶转录物和白细胞介素1 1型受体。前列腺素-内过氧化物合酶2和α-突触核蛋白的比例增加是CNS的特征。碱性磷酸酶和伊加受体的Fc片段是Pneu的特征。关于丰富的基因本体论术语，在CNS和Pneu中通常观察到“对脂多糖的应答”、“先天免疫应答”和“膜固有应答"。与“血红蛋白”和“止血”相关的丰富基因本体论术语分别是CNS和Pneu.ConclusionThese症状相关的转录本可能是A（H1N1）pdm 09感染发病机制的一些线索。
BackgroundThe pathogenic mechanisms underlying influenza A(H1N1)pdm09‐associated central nervous system (CNS) manifestations and pneumonia remain unclear. This study examined A(H1N1)pdm09 host responses using gene expression profiles of patients’ peripheral blood.MethodsSixteen A(H1N1)pdm09‐infected children in three groups were examined: a CNS group, with convulsion and altered consciousness (n= 6); a pneumonia (Pneu) group (n= 5); and a group of infected control patients (n= 5). The signal ratios of the acute to recovery phases in CNS or Pneu were analyzed versus those of the control.ResultsThe CNS (619 transcripts) and Pneu (656 transcripts) groups had significantly increased signal ratios compared to the control group. Regarding the increased ratios of transcripts shown by multiple probes, contactin‐associated protein‐like 3 transcripts, oleoyl‐ACP hydrolase transcripts, and interleukin 1 type 1 receptor were observed in CNS and Pneu. Increased ratios of prostaglandin‐endoperoxide synthase 2 and α‐synuclein were characteristic of CNS. Alkaline phosphatase and the Fc fragment of IgA receptor were characteristic of Pneu. Regarding enriched gene ontology terms, ‘response to lipopolysaccharide’, ‘innate immune response’, and ‘intrinsic to membrane’ were observed commonly in CNS and Pneu. Enriched gene ontology terms related to ‘hemoglobin’ and ‘hemostasis’ were, respectively, characteristic of CNS and Pneu.ConclusionThese symptom‐associated transcripts might be some clues to the pathogenesis of the A(H1N1)pdm09 infection.