Volumetric Strategy for Quantitatively Elucidating a Local Hydration Network around a G-Quadruplex
Volumetric Strategy for Quantitatively Elucidating a Local Hydration Network around a G-Quadruplex
复制标题
定量阐明 G-Quadruplex 周围局部水化网络的体积策略
DOI：
10.1021/acs.analchem.2c01075
复制
发表时间：
2022
影响因子：
7.4
通讯作者：
Sugimoto Naoki
中科院分区：
文献类型：
--
作者：
Matsumoto Saki;Takahashi Shuntaro;Bhowmik Sudipta;Ohyama Tatsuya;Sugimoto Naoki
核酸（如DNA和RNA）周围的水合作用不仅是核酸稳定性的重要因素，也是它们与结合分子相互作用的重要因素。因此，有必要定量地阐明核酸在一定结构周围的水化性质。在这项研究中，通过系统地改变高压下g -四重体（G4） RNA堆叠的数量，研究了g -四重体（G4） RNA形成的体积变化。在每个G4结构单元水平上的体积贡献表明，核心G4螺旋比其他部分（包括g -四重奏和环的边缘）明显脱水。这些发现将有助于预测G4配体在G4表面的结合，这取决于配体的化学结构和溶液环境。因此，预设的体积参数提供了可以预测细胞中分子拥挤过程中G4形成中的分子相互作用的信息。
Hydration around nucleic acids, such as DNA and RNA, is an important factor not only for the stability of nucleic acids but also for their interaction with binding molecules. Thus, it is necessary to quantitatively elucidate the hydration properties of nucleic acids around a certain structure. In this study, volumetric changes in G-quadruplex (G4) RNA formation were investigated by systematically changing the number of G-quartet stacks under high pressure. The volumetric contribution at the level of each G4 structural unit revealed that the core G4 helix was significantly more dehydrated than the other parts, including the edges of G-quartets and loops. These findings will help in predicting the binding of G4 ligands on the surface of G4, depending on the chemical structure of the ligand and solution environment. Therefore, the preset volumetric parameter provides information that can predict molecular interactions in G4 formations during molecular crowding in cells.