A metabolic profile in Ruditapes philippinarum associated with growth-promoting effects of alginate hydrolysates.
A metabolic profile in Ruditapes philippinarum associated with growth-promoting effects of alginate hydrolysates.
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DOI：
10.1038/srep29923
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发表时间：
2016-07-20
影响因子：
4.6
通讯作者：
Kawano S
中科院分区：
文献类型：
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作者：
Yamasaki Y;Taga S;Kishioka M;Kawano S
本研究的目的是证明藻酸盐水解物（AHs）对菲律宾马尼拉蛤的促生长作用，并验证在AHs的刺激下菲律宾蛤体内发生的生理变化。我们发现，在硅藻新细角毛藻（Chaetoceros neogracile）的饲料中添加4mg /mL的AHs可显著促进蛤蜊的生长。此外，代谢组学表明，饥饿、食物饱足和性成熟的每种状态都有一个独特的模式。在给予AHs的各组中，除了新细草外，多余的碳水化合物被积极地用于生殖组织的发育。相比之下，只给予新薄叶蛤的各组似乎在积极生长，利用它们充足的碳水化合物资源。与此同时，由于缺乏能源来源，没有食物的群体已经放慢了增长速度。因此，在藻饲料的基础上添加AHs可能是一种廉价的方法来缩短菲律宾红鲌的饲养期。此外，代谢组学可以全面评价菲律宾蛤的生长状况，这不仅对开发大量养殖方法，而且对蛤资源的野外保护具有重要意义。
The aim of this study is to demonstrate the growth-promoting effect of alginate hydrolysates (AHs) on the Manila clam Ruditapes philippinarum, and to verify the physiological change occurring within a living R. philippinarum stimulated by AHs. We show that growth of clams was dramatically promoted by supplementing a diet of the diatom Chaetoceros neogracile with AHs at 4 mg/mL. Furthermore, metabolomics indicates that each state of starvation, food satiation, and sexual maturation have a characteristic pattern. In the groups given AHs in addition to C. neogracile in particular, excess carbohydrate was actively utilized for the development of reproductive tissue. In contrast, it appeared that clams in the groups given C. neogracile only were actively growing, utilizing their adequate carbohydrate resources. Meanwhile, the unfed groups have slowed growth because of the lack of an energy source. Hence, supplementation of AHs in addition to the algal diet may be an inexpensive way to shorten the rearing period of R. philippinarum. Moreover, metabolomics can evaluate the growth condition of R. philippinarum in a comprehensive way, and this approach is crucially important for not only the development of a mass culture method but also for the conservation of the clam resource in the field.