Effect of a conjugated acridine moiety on the binding and reactivity of Cu(II)[9-acridinylmethyl-1,4,7-triazacyclononane] with DNA
Effect of a conjugated acridine moiety on the binding and reactivity of Cu(II)[9-acridinylmethyl-1,4,7-triazacyclononane] with DNA
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DOI：
10.1016/j.jinorgbio.2004.07.014
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发表时间：
2004-11-01
影响因子：
3.9
通讯作者：
Chikira, M
中科院分区：
文献类型：
--
作者：
Hirohama, T;Arii, H;Chikira, M
通过 DNA 纤维 EPR（EPR = 电子顺磁共振）光谱研究了 1,4,7-三氮杂环壬烷 ([9]aneN(3)), 1 和吖啶缀合物 2 的 Cu(II) 复合物的 DNA 结合方向和动态行为。通过X射线衍射测定了2的晶体和分子结构。研究表明，1 在室温下以两种不同的模式与 DNA 结合；一种物种快速旋转，另一种则随机固定在 DNA 上。将吖啶引入[9]aneN(3)将2的[Cu([9]aneN(3))](2+)部分固定在DNA上的两种不同环境中：一种物质的g（平行于）轴（g（平行于）= 2.26）垂直于DNA纤维轴对齐，而另一种物质的g（平行于）= 2.24）对齐
The DNA binding orientation and dynamic behavior of Cu(II) complexes of 1,4,7-triazacyclononane ([9]aneN(3)), 1, and an acridine conjugate, 2, were investigated by DNA fiber EPR (EPR = electron paramagnetic resonance) spectroscopy. Crystal and molecular structure of 2 were determined by X-ray diffraction. It has been shown that 1 binds to DNA in two different modes at room temperature; one species is rapidly rotating and the other is immobilized randomly on the DNA. The introduction of acridine to [9]aneN(3) fixed the [Cu([9]aneN(3))](2+) moiety of 2 in two different environments on the DNA: the g(parallel to) axis of one species (g(parallel to) = 2.26) is aligned perpendicularly to the DNA fiber axis whereas that of the other (g(parallel to) = 2.24) aligns