Protease Deficiency and Its Association with Defects in Spore Coat Structure, Germination and Resistance Properties in a Mutant of Bacillus subtilis
Protease Deficiency and Its Association with Defects in Spore Coat Structure, Germination and Resistance Properties in a Mutant of Bacillus subtilis
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枯草芽孢杆菌突变体中蛋白酶缺乏及其与孢子衣结构、萌发和抗性特性缺陷的关系
DOI：
10.1099/00221287-129-9-2727
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发表时间：
1983
期刊：
影响因子：
1.5
通讯作者：
H. Lord
中科院分区：
文献类型：
--
作者：
H. Jenkinson;H. Lord
摘要：枯草芽孢杆菌携带突变ger-36形成的孢子对溶菌酶敏感且萌发缺陷。这些孢子的外壳缺乏通常在孢子外壳中发现的一些多肽，并且存在四种在野生型孢子外壳中通常不存在的额外多肽。ger-36突变还阻止胞内蛋白酶（蛋白酶B，约为5期）的合成（约为5期）和向孢子外层的掺入。单体的Mol wt（25000）。分离出一株携带ger-36的部分逆转菌株，它在与gerE不同的位点上有一个额外的突变。抑制突变恢复了蛋白酶活性，但没有恢复萌发缺陷。部分恢复了对溶菌酶的抗性，形成的孢子除了缺少一个多肽（mol wt. 36000）外，外壳蛋白组成明显正常。
Summary: Spores formed by Bacillus subtilis carrying the mutation ger-36 are lysozyme-sensitive and germination-defective. The coats of these spores lack a number of polypeptides normally found in the spore coat, and four additional polypeptides are present that are not normally found in the coats of wild-type spores. The ger-36 mutation also prevents the synthesis (at about stage V) and the incorporation into the spore outer layers, of an intracellular protease (protease B, approx. mol wt. of monomer 25000). A partial revertant of a strain carrying ger-36 was isolated and this had an additional mutation in a locus distinct from gerE. The suppressing mutation restored the protease activity, but not the germination defect. It partly restored resistance to lysozyme and the spores formed had apparently normal coat protein composition except for the absence of one polypeptide (mol wt. 36000).